中文摘要：

目的：观察天王补心丸四个SP825大孔吸附树脂洗脱部分的镇静催眠作用。 方法：实验于2005—06／08在军事医学科学院放射与辐射医学研究所药理毒理研究室完成。健康昆明种小鼠200只，随机数字表法分为空白对照组，阳性药组，水洗脱部位组，体积分数为0．2乙醇洗脱部位组，体积分数为0．5乙醇洗脱部位组，体积分数为0．8乙醇洗脱部位组，每组10只。空白对照组给予等体积生理盐水，阳性药组给予阳性药，其余四个受试药物组分别给予天王补心丸SP825洗脱物。①协同睡眠试验：各组末次给药后30min，腹腔注射戊巴比妥钠40mg／kg，以小鼠翻正反射消失达1min以上为入睡指标，记录睡眠时间。②自发活动试验：分别于各组末次给药30min、90min两个时间点观测小鼠自发活动，记录5min的自发活动次数。③抗惊厥试验：各组末次给药后1h，腹腔注射戊四唑溶液（65mg／kg），以小鼠前肢和头部发生阵挛性抽搐为指标，记录惊厥潜伏期。 结果：实验大鼠200只均进入结果分析。①协同睡眠试验结果：天王补心丸SP825体积分数为0．2乙醇洗脱部分能够明显延长阈剂量戊巴比妥钠引起小鼠睡眠的时间（P＜0．05）。②自发活动试验结果：给药后30min，各受试药物组的自发活动与对照组相比均无显著性差异（P＞0．05）；给药后90min，天王补心丸SP825体积分数为0．5乙醇洗脱部分能够明显减少小鼠的自发活动次数（P＜0．05）。③抗惊厥试验结果：天王补心丸SP825体积分数为0．2和0．5乙醇洗脱部分均能够明显延长戊四唑所致小鼠阵挛性抽搐的潜伏期（P＜0．05）。 结论：天王补心丸镇静催眠的活性部位是其亲水性较强的SP825体积分数为0．2和0．5乙醇洗脱部分。

英文摘要：

AIM： To observe the sedative and hypnotic effects of four SP825 macroporous resins diffluences of tianwang buxin wan. METHODS： The experiment was conducted in the Pharmacology and Toxicology Laboratory, Institute of Radiation Medicine, Academy of Military Medical Sciences from June to August 2005. 200 healthy Kunming mice were randomly assigned into six groups： blank control group, positive drug group, water diffluence from tianwang buxin wan group and volume fraction 0.2, 0.5 and 0.8 alcohol diffluence groups with 10 mice in each group. The blank control group was given matching normal saline; the positive drug group was given the positive drug; the other 4 groups were given SP825 macroporous resins diffluences of tianwang buxin wan, respectively. ①Pentobarbital-induced sleeping time： The mice in each group were intraperitoneally injected 40 mg/kg pentobarbital sodium 30 minutes after the last administration; the body-righting reflex disappeared for more than 1 minutes served as the sleeping index to record the sleeping time. ② Spontaneous motor activity： The spontaneous motor activity of mice was observed at 30 and 90 minutes after the last administration to record the activity in 5 minutes. ③Pentylenetetrazole induced eclampsia： One hour after the last administration, the mice were intraperitoneally injected pentylenetetrazole （65 mg/kg）; the eclampsia latency was recorded with the clonic convulsion of forward limb and head as the index. RESULTS： All the 200 mice were involved in the result analysis. ①Pentobarbital-induced sleeping time： SP825 alcohol with volume fraction 0.2 of tianwang buxin wan could significantly prolong the sleeping time induced by threshold doses pentobarbital sodium （P 〈 0.05）.②Spontaneous motor activity： 30 minutes after administration, no significant difference was found among the four test drugs groups and the control group （P 〉 0.05）; 90 minutes after administration, SP825 0.5 alcohol could markedly reduced the spontaneous motor activity ?