中文摘要：

目的探讨泥蚶多糖佐剂对日本血吸虫DNA疫苗免疫效果的影响。方法将60只雌性BALB/c小鼠随机分为对照组、泥蚶多糖组、疫苗组、疫苗＋佐剂组等4组,每组15只,分别皮下注射100μl PBS、100μg泥蚶多糖、100μg pc DNA3.1-Sj26GST血吸虫DNA疫苗、100μg pc DNA3.1-Sj26GST与等量泥蚶多糖佐剂的混合物。各组分别于实验开始时、第2周、第4周各免疫1次。末次免疫后第2周,以日本血吸虫尾蚴40±1条/只经腹部皮肤攻击感染小鼠。感染后6周剖杀小鼠,收集血清、肝脏及成虫,以ELISA法测定血清中特异性Ig G水平,计算减虫率及每克肝组织减卵率。结果感染后6周,疫苗组和疫苗＋佐剂组小鼠血清中Ig G抗体水平分别为18.26±0.42 mg/ml和20.21±0.89 mg/ml,差异有统计学意义（P〈0.05）,两组均显著高于对照组（P均〈0.05）;疫苗组减虫率和减卵率分别为28.60%和35.84%,疫苗＋佐剂组减虫率和减卵率分别为38.04%和49.74%,差异均有统计学意义（P均〈0.05）,且均显著高于PBS对照组（P均〈0.01）。结论泥蚶多糖作为佐剂,对日本血吸虫DNA疫苗具有明显增效作用。

英文摘要：

Objective To discuss the effect of polysaccharides from Tegillarca granosa as an adjuvant on pc DNA3.1-Sj26 GST vaccine against Schistosoma japonicum infection. Methods Sixty SPF BALB/c mice were divided into four groups randomly（15 mice each group）,including a control group,a polysaccharides group,a vaccine group,and a vaccine plus polysaccharides group. In the 0,2th and 4th week of the experiment,the mice in the above four groups were immunized for 3 times with 100 μl PBS,100 μg polysaccharides from Tegillarca granosa,100 μg Sj26 GST vaccine,and 100 μg Sj26 GST vaccine plus equivalent polysaccharides,respectively. Two weeks after the last immunization,all the mice were infected with 40 ± 1Schistosoma japonicum cercariae through the skin of the abdomen. After the infection for 6 weeks,all the mice were sacrificed,and their serums,livers and the adult worm of Schistosoma japonicum in them were collected,the specific sera Ig G antibodies were detected by ELISA,and the worm reduction rates and egg reduction rates in the liver were calculated. Results Six weeks after the infection,the Ig G antibody levels of the mice in the vaccine group and the vaccine plus polysaccharides group were18.26 ± 0.42 mg/ml and 20.21 ± 0.89 mg/ml respectively,the difference between them were statistically significant,and both of the Ig G levels of the above groups were significantly higher than those in the control（both P 〈0.05）. The worm reduction rate and egg reduction rate in the vaccine group were 28.60% and 35.84%,respectively,and the rates in the vaccine plus polysaccharides group were 38.04% and 49.74%,respectively,the differences between the worm reduction rates and egg reduction rates were both statistically significant（both P 〈0.05）. Meanwhile,the two rates in the two above groups were all significantly higher than those in the control group（all P 〈0.01）. Conclusion Polysaccharides from Tegillarca granosa using as an adjuvant can increase the protection effect of pc DNA3.1-Sj26 GST vaccine against S