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中华倒刺鲃线粒体细胞色素b基因的遗传多样性分析
  • ISSN号:1004-874X
  • 期刊名称:《广东农业科学》
  • 时间:0
  • 分类:Q959.468[生物学—动物学]
  • 作者机构:[1]暨南大学水生生物研究所,广东广州510632
  • 相关基金:国家自然科学基金(41071034); 暨南大学科研培育与创新基金(21611426)
中文摘要:

测定了长江支流贵定与干流合江和宜都3个群体57尾中华倒刺鲃细胞色素b基因5'端971 bp序列以分析其遗传多样性和种群结构。结果发现21个变异位点和13个简约信息位点,检测到14种单倍型,平均单倍型多样性(Hd)和核苷酸多样性(Pi)分别为0.7820和0.0025,表现出较低的遗传多样性。合江群体核苷酸多样性最高(0.0036),宜都其次,贵定最低。在NJ系统树上没有出现明显的谱系结构和地理聚群,AMOVA分析显示遗传变异主要集中在群体内的个体间(61.16%)。宜都和合江群体间的Fst和Nm值分别为0.0974和4.6329,但贵定群体与合江和宜都群体间的Fst值分别为0.4549、0.4875,Nm值分别为0.5991、0.5256,表明长江干流2个地理群体间遗传分化程度低,可视为一个大的随机交配群体;而支流群体没有遗传变异,且与长江干流群体间出现高度分化,可能是由贵定特殊地理条件使基因交流受阻所致。中性检测表明,宜都群体在约为7.6万-3.0万年前的更新世晚期发生过种群的快速扩张。

英文摘要:

The genetic diversity and population genetic structure of Spinibarbus sinensis were examined by sequence analysis of a 971 bp fragment of the 5'-end of the cytochrome b gene in 57 fish collected from tributary Guiding population and mainstream Hejiang and Yidu populations in the Yangtze River drainage.Totally 21 variable sites and 13 parsimony informative sites were found in 14 haplotypes,and the average haplotype diversity(Hd) and nucleotide diversity(Pi) were 0.7820 and 0.0025 respectively,indicating a poor genetic diversity.The highest nucleotide diversity were found in Hejiang population(0.0036),followed by Yidu population(0.0025),and Guiding population had no genetic variation.No obvious lineages and geographic clusters were found in the Neighbor-joining tree,and 61.16% genetic variation occurred within populations in AMOVA analysis.As Fst and Nm between Hejiang and Yidu populations were 0.0974 and 4.6329 respectively,while the Fst and Nm between Guiding population and Hejiang and Yidu populations were 0.4549 and 0.4875,0.5991 and 0.5256 respectively,indicating that genetic differentiation between the two mainstream populations were low,and they could be treated as a single panmictic population.No genetic variation and highly differentiated tributary population might due to the failure to gene flow caused by its special geographical conditions.Neutral analysis suggested that Yidu population had a late Pleistocene rapid population expansion at about.

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期刊信息
  • 《广东农业科学》
  • 北大核心期刊(2011版)
  • 主管单位:广东省农业科学院
  • 主办单位:广东省农业科学院 华南农业大学
  • 主编:
  • 地址:广州市天河区金颖路31号五山广东省农科院农经所
  • 邮编:510640
  • 邮箱:gdnykx@vip.163.com
  • 电话:020-38319948 38319941 38319942 38319946
  • 国际标准刊号:ISSN:1004-874X
  • 国内统一刊号:ISSN:44-1267/S
  • 邮发代号:
  • 获奖情况:
  • 广东省第二、第三届优秀科技期刊,广东省首届十佳期刊,中国期刊方阵“双效”期刊,第四届全国农业优秀期刊一等奖,第五届全车优秀农业期刊鑫犁奖一等奖,首届全国CAD规范一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国动物学记录,中国北大核心期刊(2008版),中国北大核心期刊(2011版)
  • 被引量:33995