中文摘要：

目的观察运动训练对发育期大鼠反复惊厥所致远期认知损伤及海马Zn^2＋转运体3（ZnT-3）表达水平的影响。方法将出生21d的SD大鼠随机分成空白对照组、运动对照组、单纯惊厥组及惊厥运动组，每组10只。采用青霉素腹腔注射方法诱发单纯惊厥组及惊厥运动组大鼠反复惊厥，空白对照组及运动对照组大鼠则同期腹腔注射等量生理盐水，运动对照组和惊厥运动组于出生后48～53d期间进行踏转轮训练。各组大鼠分别于出生后39～44d及61～65d期间进行Morris水迷宫测试，于出生后66d时采用实时定量逆转录聚合酶链反应（RT-PCR）技术检测ZnT-3在海马中的表达情况。结果①搜寻策略比较：各组大鼠边缘式搜寻比例均呈逐渐减少趋势，而趋向式及直线式搜寻比例均呈逐渐增加趋势。第1阶段Morris水迷宫测试发现两对照组成绩在第1天及第4天时均明显优于两惊厥组（均P〈0．05）；第2阶段Morris水迷宫测试发现运动对照组及惊厥运动组搜寻策略成绩在第2天以后，均显著优于空白对照组及单纯惊厥组（P〈0．05）。②记忆功能比较：在前、后2阶段Morris水迷宫测试中，两惊厥组大鼠穿越原平台所在区域的次数均明显少于两对照组（均P〈0．05），且单纯惊厥组与惊厥运动组间差异无统计学意义（P〉0．05）。③实时定量RT—PCR结果比较：惊厥运动组大鼠海马ZnT-3 mRNA表达水平均明显高于两对照组及单纯惊厥组（均P〈0．05）。结论青霉素诱发反复长程惊厥能导致发育期大鼠学习、记忆功能远期损伤，运动训练能明显改善受损学习功能，其治疗机制可能与上调海马ZnT-3表达水平有关。

英文摘要：

Objective To explore the effects of physical exercises on recurrent seizures-induced long-term learning and memory deficits and on the expression of zinc transporter-3 （ZnT-3） in hippoeampuses of developmental period rats. Methods Sprague-Dawley rats aged 21 d were randomly divided into blank control group, exercises control group, seizure group and seizure plus exercises group. Abdominal cavity injections of penicillin or saline were used to induce recurrent seizure or as a control in the corresponding groups. During the postnatal （P） 39-44 d and P61-65 d periods, Morris water-maze tests were administered to evaluate spatial learning and memory capacity. During the P48-53 d period, the rats in exercises control and seizure plus exercises groups were subjected to a 30-min daily aerobic exercises program for 6 d. The real-time reverse transcription-polymerase chain reaction （RT-PCR） method was used to detect the expression of ZnT-3 mRNA in hippocampuses of all rats at P66 d. Results Searching strategy： There was a decreasing incidence of marginal strategy and an increasing trend in the use of taxis and straight line strategy in all four groups. The scores on d 1 and 4 were significantly higher in two control groups than in two seizure groups in water-maze test （ all P 〈 0.05）. By d 2 the exercises control and seizure plus exercises groups were scoring significantly higher than the bland control and seizure groups （ P 〈 0.05 ）. Memory test ： The frequency of passing through the platform quadrant decreased significantly in the two seizure groups compared to the two control groups in both probe tests （ all P 〈 0.05 ）. RT-PCR test ： ZnT-3 mRNA expressions in hippocampuses were significantly higher in seizure plus exercises group than in any other groups. Conclusions Penicillin-induced recurrent seizures can induce long-term damage on learning and memory capacity in developmerital period rats. Physical exercises can improve learning capacity. It＇s mechanism may be velated to the up