中文摘要：

为了满足实验室测试和野外测试需要,也为其他学者研究不同抗生素之间的相互作用提供相关的资料,本研究选用环丙沙星（CIP）和氟甲喹（FLU）作为代表,建立了一种简单、稳定、易普及的高效液相色谱法用于同时快速测定水体中两种氟喹诺酮类抗生素的含量,并讨论了不同流动相及其比例和水样中几种常见阴、阳离子（Ca2＋、Mg2＋、Fe3＋、Al3＋、SO24-和HCO3-）对抗生素测定的影响。结果表明：三乙胺对改善柱效有明显效果;低浓度离子对测试影响不大,但Fe3＋和Al3＋可能与固定相的表面羟基或测试组分发生配合作用,造成基线不稳。实验结果对其他研究者对于流动相的选择与优化具有借鉴意义。

英文摘要：

In order to meet the field sample measurements and laboratory research,a simple,stable,and popular high performance liquid chromatographic（HPLC） method was established for the determination of the concentrations of two fluoroquinolone antibiotics in water samples simultaneously and rapidly.Thus,it could provide valuable information for other scholars to study the interactions between different antibiotics.Ciprofloxacin（CIP） and flumequine（FLU） were taken as the target contaminants because of their wide application in medical career.Furthermore,this study also investigated the different types of mobile phase,different mobile phase ratios,common ions（Ca2＋,Mg2＋,Fe3＋,Al3＋,SO2-4and HCO-3） in water samples and other factors on CIP and FLU measurements with this method.The results showed that triethylamine has obvious effect on improving column efficiency.Low concentrations of ions had little effect on the test,but Fe3＋and Al3＋might cause baseline instability,because Fe3＋and Al3＋may form complexes with the surface hydroxyl groups of the stationary phase or the test components.The results may be referred by other workers for the optimization of the determination conditions.