中文摘要：

目的：通过观察益气解毒活络中药复方对早期糖尿病肾病（DN）大鼠肾组织单核细胞趋化蛋白-1（MCP-1）mRNA和肿瘤坏死因子-α（TNF-α）mRNA表达的影响,以探讨益气解毒活络中药复方防治早期糖尿病肾病的效果及其作用机制,并深入探讨早期糖尿病肾病发生发展的病理机制。方法：将实验用大鼠分为正常对照组、模型组、中药复方预防组、中药低剂量治疗组、中药高剂量治疗组及阳性西药对照组,采用高糖高脂饲料喂养结合一次尾静脉注射链脲佐菌素（STZ）的造模方法复制出糖尿病肾病模型,各药物组干预4周后取肾脏,用Real-time PCR法测试各组MCP-1 mRNA和TNF-αmRNA的表达情况,最后进行组间比较。结果：模型组大鼠MCP-1mRNA和TNF-αmRNA含量均比正常对照组明显上升（P〈0.01）,经药物干预后,各组大鼠上述两项指标的mRNA含量均比模型组显著降低（P〈0.01）。结论：益气解毒活络中药复方防治DN发生发展的药理机制可能是通过降低MCP-1 mRNA和TNF-αmRNA的高表达来实现;DN的发病机制可能与肾组织中MCP-1 mRNA和TNF-αmRNA的异常表达密切相关。

英文摘要：

Objective：To explore the effect and mechanism of treatment of invigorating vital energy,detoxicating and activating collaterals to the early period diabetic nephropathy by observing mRNA expression of MCP-1 and TNF-αin kidney tissue of DN Rats.Methods：The rats were divided into 6 groups including normal control group,model blank group,prophylaxis group,LD group,HD group and positive control group.Build up the DN model by HG HF feeding and once injection through vena caudalis with STZ.Rats in every group accepted the action of every required interference factor for 4 weeks,and then executed to death and kidney tissue obtained.Measured the expression of MCP-1 and TNF-α by Real-time PCR and then compared among groups.Results：Expression of MCP-1 and TNF-α in model blank group were significantly higher than that in normal control group（P0.01）,after intervention of drugs of other groups rats showed signicantly lower than model blank group（P0.01）.Conclusion：Prescription of invigorating vital energy,detoxicating and activating collaterals probably prevent and cure DN by regulating the abnormal expression of MCP-1 and TNF-α in kidney tissue.The pathogenesy of DN may be closely related to the abnormal expression of MCP-1 and TNF-α in kidney tissue.