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一株产吲哚菌的分离鉴定及其碳源发酵特性分析
  • ISSN号:1000-2030
  • 期刊名称:《南京农业大学学报》
  • 时间:0
  • 分类:Q939.96[生物学—微生物学]
  • 作者机构:[1]南京农业大学消化道微生物研究室,江苏南京210095
  • 相关基金:国家973计划项目(2004CB117500-4)
中文摘要:

利用含L-色氨酸的基础培养基从仔猪新鲜粪样中分离产吲哚的菌株,根据菌落形态、生理生化特征分析,鉴定该菌株为Escherichia属,命名为wz_1977。通过批次培养的方法,研究了wz_1977以不同碳源为底物时的生长和吲哚生成情况。结果表明:以葡萄糖为底物时,wz_1977的菌落数目显著高于其他各组(P〈0.05),而吲哚生成水平最低;以半乳寡糖为底物时,wz_1977的菌落数目仅次于葡萄糖,而吲哚生成水平高于葡萄糖组;以果寡糖为底物时,wz_1977的菌落数目与对照组(不含碳源)相比没有显著差异(P〉0.05),而吲哚生成水平稍低于对照组(P〈0.05)。结论:分离出1株产吲哚菌wz_1977,该菌的发酵模式为:以葡萄糖为底物时以合成菌体蛋白为主,以果寡糖为底物时以生成吲哚为主。

英文摘要:

An indole-producing bacterium named wz_1977 was isolated from fresh faeces of piglets by enrichment in basal medium supplemented with L-tryptophan,and the strain was identified as Escherichia sp.based on its morphology,biochemistry test and 16S rRNA sequencing analysis.We also investigated its characteristics of the growth and indole production with glucose(GLU),galactooligosaccharides(GOS)and fructooligosaccharides(FOS)as substrate respectively.The results indicated that the viable counts did not differ between the control(no carbohydrate)and FOS group,whereas indole concentrations in FOS group were lower than those in control group(P0.05).With GOS as substrate the viable counts of strain wz_1977 were lower and indole levels were higher when compared to GLU group,in which the viable counts were highest with the lowest levels of indole production(P0.05).These results showed that a strain of indole-producing bacterium was isolated from faeces of piglets and in vitro study tryptophan was used for bacterial protein synthesis with GLU as substrate but for indole production with FOS as substrate.

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期刊信息
  • 《南京农业大学学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国教育部
  • 主办单位:南京农业大学
  • 主编:郑小波
  • 地址:江苏省南京市卫岗1号
  • 邮编:210095
  • 邮箱:nauxb@njau.edu.cn
  • 电话:025-84395214
  • 国际标准刊号:ISSN:1000-2030
  • 国内统一刊号:ISSN:32-1148/S
  • 邮发代号:28-53
  • 获奖情况:
  • 中国自然科学核心期刊,全国优秀科技期刊,第二届江苏省十佳科技期刊,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:22558