中文摘要：

目的：建立一种麦冬多糖中单糖组成的高效液相色谱（HPLC）-蒸发光散射（ELSD）测定方法。方法：以三氟乙酸（TFA）水解麦冬多糖，采用WatersXBridge^TM Amide色谱柱，乙腈-水-三乙胺（75：25：0．2）为流动相，ELSD检测器监测麦冬多糖的酸水解产物，优化麦冬多糖的全水解条件，并对麦冬多糖的单糖组成进行分析。结果：麦冬多糖由果糖和葡萄糖组成，其全水解条件为以0．02mol·L^-1 TFA为水解试剂，在110℃下水解2h。HPLC—ELSD方法验证结果表明，该单糖组成测定方法的精密度和重复性良好，果糖和葡萄糖的加样回收率分别为95．9％-104．4％和91．3％-104．1％（RSD〈5．9％）。麦冬多糖中果糖和葡萄糖的摩尔比约为15：1。结论：本研究为麦冬多糖中单糖组成的准确测定提供了方法。

英文摘要：

Objective：To develop a method for determination of monosaccharide composition in Ophiopogon japoni- cus polysaccharides（OJP） by high performance liquid chromatography coupled with evaporative light scattering de- tector （ HPLC - ELSD）. Methods： OJP was hydrolyzed by trifluoroacetic acid （TFA） solution, and the acid hydrolysis products were separated by a Waters XBridgeTM Amide column, using acetonitrile - water - triethylamine （75： 25： 0. 2 ） as a mobile phase. ELSD detector was utilized to monitor the acid hydrolysis products of OJP. The full optimized hy- drolysis conditions were investigated and monosaccharide compositions in OJP were finally determined by HPLC - ELSD. Results： The OJP was composed of fructose and glucose, and the full hydrolysis was conducted at 110 ℃ for 2 h with 0.02 mol . L-1 TFA as hydrolysis reagent. The validation results of the HPLC - ELSD method showed that both precision and repeatability of the hydrolysis and determination method for monosaccharide composition were good. The recoveries of fructose and glucose were 95.9% - 104.4% and 91.3% - 104.1% （with RSD less than 5.9% ）, respectively. The molar ratio of fructose and glucose in OJP was about 15： 1. Conclusion：This study pro- vides an accurate method for monosaccharide composition determination in OJP.