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中文摘要：
目的：建立一种快速、准确的大黄鱼小黄鱼实时荧光PCR鉴定方法.方法：根据GenBank中大黄鱼、小黄鱼线粒体中ND6基因的序列,设计并合成特异性引物和cycling探针,以草鱼为阴性对照,进行实时荧光PCR反应.结果：利用设计合成的大黄鱼和小黄鱼特异性引物和cycling探针进行实时荧光PCR反应,大黄鱼和小黄鱼均产生特异扩增曲线,对照的草鱼没有产生扩增曲线.结论：利用设计的引物和cycling探针,可准确快速鉴定大黄鱼和小黄鱼.
英文摘要：
Aim：Establish a rapid and accurate real-time PCR method to identify Pseudosciaena crocea and Pseudosciaena polyatics. Methods：Specific primers and cycling probe were designed and synthesized according to ND6 gene sequence in the mitochondria of Pseudosciaena crocea and Pseudosciaena polyatics, and the negative control was Ctenopharyngodon idellus. They were detected by the reaction of Real-time PCR. Results：Specific amplification plots were generated in reactions of Real-time PCR with the mitochondrial DNA of Pseudosciaena crocea and Pseudosciaena polyatics, and was not with Ctenopharyngodon idellus. Conclusion ： Pseudosciaena crocea and Pseudosciaena polyatics can be quickly and accurately identified by Real-time PCR with designed primers and cycling probe.
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