中文摘要：

目的：探讨甲基乙二醛（MGO）对胰腺癌 PANC-1细胞增殖影响及其作用机制。方法培养PANC-1细胞，用四甲基偶氮唑蓝（MTT）比色法检测MGO对PANC-1细胞增殖的影响；Hoechst33258染色细胞后显微镜下观察MGO处理后PANC-1细胞形态变化，Western blot检测凋亡家族蛋白Bcl-2、Bax和Caspase-3的表达变化。结果 MTT检测发现MGO对人胰腺癌PANC-1有明显的抑制作用，且在一定程度上呈剂量时间依赖性；MGO处理48 h后的PANC-1细胞在Hoechst33258染色后荧光显微镜下呈现典型的凋亡细胞核固缩表现；Western blot检测表明MGO能显著降低Bcl-2蛋白表达量而提高Bax和Caspase-3蛋白表达量。结论 MGO能够有效抑制人胰腺癌PANC-1细胞增殖并诱导其凋亡，其作用机制可能是通过作用凋亡信号通路调控凋亡家族蛋白Bcl-2，Bax 和Caspase-3表达进而诱导PANC-1细胞凋亡。

英文摘要：

Objective To study the influence of methylglyoxal on human pancreatic carcinoma PANC-1 cells proliferation and possible mechanism. Methods Cell proliferation was evaluated using MTT method;Hoechst33258 staining assay was used to observe MGO-induced morphological changes;Changes for Bcl-2 and Bax expression level were assessed using Western blot. Results Comparing with control group, PANC-1 cells proliferation was suppressed by methylglyoxal. The growth inhibition induced by MGO was in a dose-dependent manner（P〈0.01）. Apoptosis of the PANC-1 cells was observed after exposure to MGO, which was verified by morphological changes and increased proportion of Hoechst positive cells. The expression level of Bcl-2 was significantly decreased compared with the control group, but that of Bax was higher in the former than that in the latter, as well as the expression level of Caspase-3. Conclusion MGO could inhibit PANC-1 cells proliferation significantly and induce the apoptosis via regulating the expression level of Caspase-3 and Bcl-2 family proteins.