中文摘要：

目的原代培养大鼠脑微血管内皮细胞及星形胶质细胞，在体外探讨不同剂量缓激肽的作用靶细胞。方法细胞原代培养成功后，运用免疫荧光测定脑血管内皮细胞、星形胶质细胞及C6胶质瘤细胞在不同剂量缓激肽作用前后的细胞内钙离子变化，根据给药前后的荧光改变来确定大、小剂量缓激肽的作用靶点细胞。结果小剂量缓激肽可以引起肿瘤细胞内的钙离子水平升高。而只有大剂量缓激肽才能触发星形胶质细胞内的钙离子水平变化，脑微血管内皮细胞对大、小剂量缓激肽均无任何反应。结论缓激肽的直接作用靶点是胶质细胞及肿瘤细胞，缓激肽调节脑血管内皮细胞通透性的作用可能需要某些细胞间信使的参与。

英文摘要：

Objective To investigate the target cells of bradykinin （BK） and the mechanism of BK selectively modulating the blood tumor/brain barrier. Methods By using immunofluorescence of Fu- ra-3, the intracellular calcium changes in astrocytes, C6 and brain microvascular endothelial cells （BMEC） before and after the treatment with BK at different concentrations were studied respectively to identify the target cells. Results Small dose of BK could trigger intraceUular calcium elevation in C6 cells, whereas only large dose of BK could do that in astrocytes, and the BMEC remains unresponsive to BK. Conclusion The direct target ceils of BK are astrocytes and tumor cells. BK can regulate the permeability of the BMEC mediated by some intercellular messengers.