中文摘要：

采用长距PCR扩增及保守引物步移法测定并注释了郑氏比蜢（Pielomastax zhengi）的线粒体基因组全序列。郑氏比蜢的线粒体基因组全长15602 bp,A＋T含量为71.8%,37个基因位置与飞蝗的一致, 基因间隔序列共计10处47bp, 间隔长度从1～20bp不等; 有14对基因间存在52bp重叠, 重叠碱基数在1～8bp之间。蛋白质基因的起始密码子均为昆虫典型的起始密码子ATN。ND5基因使用了不完全终止密码子T,其余基因均为典型的TAA或TAG。除tRNASer（AGN）的DHU臂缺失外, 其余21个tRNA基因的二级结构均属典型的三叶草结构, 但在郑氏比蜢中有5个tRNA（tRNACys、tRNALys、 tRNAPhe、 tRNAPro tRNAArg）基因变异较大, 无法采用常规算法预测出来, 表现在这5个tRNA二级结构的TψC臂仅有3～4对配对碱基, tRNALys 和 tRNAArg的反密码臂仅有 4 对配对碱基。预测的lrRNA二级结构总共有6个结构域（结构域Ⅲ缺失）, 44个茎环结构。预测的srRNA的二级结构包含3个结构域, 30个茎环结构。比较郑氏比蜢、西藏飞蝗（Locusta migratoria tibetensis）和疑钩额螽（Ruspolia dubia）rRNA二级结构后,发现郑氏比蜢与西藏飞蝗的更相似。A＋T丰富区中存在一个被认为与复制及转录起始有关的Ploy（T）结构。

英文摘要：

The complete mitochondrial genome sequence of Pielomastax zhengi was determined by using long PCR and conserved primer walking approaches.The results showed that the entire mitochondrial genome of Pielomastax zhengi is 15 602 bp long with A＋T content of 71.8%.All 37 genes are conserved in the positions observed in those of Locusta migratoria.All the genes are closely assembled by leaving 10 intergenic spacers in between.Those intergenic spacers are 47 bp in total（excluding the A＋T rich region）,with individual size ranges from 1 bp to 20 bp.In addition,there are a total of 52 bp overlapping sequences among 14 genes,ranging from 1-8 bp.All protein-coding genes start with a typical initiation codon in insects,ATN.Twelve protein-coding genes use the usual TAA and TAG termination codons,whereas,the ND5 genes have an incomplete termination codon（T）.Except the tRNASer（AGN）,whose DHU arm is absent;all the other 21 tRNA genes have typical clover-leaf secondary structures.But in Pielomastax zhengi,the secondary structures of five tRNA（tRNACys,tRNALys,tRNAPhe,tRNAPro,tRNAArg） genes can not be predicted by the conventional methods as they have only 3-4,rather than 5 base pairs in the TψC arm,while,the tRNALys and tRNAArg have only 4 base pairs in the anticode arm.The predicted secondary structures of lrRNA and srRNA have 6 domains with 44 helices and 3 domains with 30 helices,respectively.The results of the rRNA secondary structure comparison showed that Pielomastax zhengi is more closely related with Locusta migratoria tibetensis than Ruspolia dubia.Like most insects,the mitochondrial genome of Pielomastax zhengi has a non-coding A＋T rich region containing a polythymidine stretch,which may be involved in the replication and/or translation initiation of other genes.