中文摘要：

目的 探讨孕妇人群显性和隐匿性乙型肝炎病毒（HBV）感染及其HBV病毒株S基因的分子进化特征。方法 2014年6月1日—31日,在知情同意下,连续招募安徽省安庆市立医院产科初次住院活胎孕妇156人,收集其人口学特征和血清;对所有血清平行进行乙型肝炎病毒表面抗原（HBsAg）酶联免疫吸附法和电化学发光法检测、应用实时PCR定量测定HBV DNA、应用巢式PCR进行HBV S片段扩增、测序及进化分析。结果156名孕妇中,显性和隐匿性HBV感染率分别为10.3%（16/156）、8.6%（12/140）;成功获得13株HBV S基因序列,其中显性7株,隐匿性6株;所有病毒株均为B基因型;仅1例隐匿性HBV株为ayw1血清型,其余12株均为adw2血清型。结论 孕妇人群携带较高比例的显性和隐匿性HBV。

英文摘要：

Objective To explore the prevalence and S region characteristics of isolated hepatitis B virus （HBV） among pregnant women with overt and occult HBV infection. Methods A cross-sectional study was conducted among 156 pregnant women recruited continuously from June 1,2014 to June 3l ,2014 in a hospital in Anqing city of Anhui province. Blood samples were collected and detected for HBV surface antigen（HBsAg）by using enzyme linked immu- nosorbent assay （ELISA） and electrochemiluminescence immunoassay. Real-time PCR was applied to quantify HBV DNA. Nested polymerase chain reaction and sequencing of HBV S gene were also performed for all samples. Phylogenetic analysis was performed using MEGA 6. 0 software. Results The prevalence HBV infection was 17.95 % （28/156） and the proportions of overt and occult infection were 10. 3% （ 16/156 ） and 8.6% （ 12/140 ）, respectively, among the pregnant women. S gene sequences were obtained for 13 HBV strains isolated from 7 overt and 6 occult HBV infections. All the isolated HBV strains were serotype adw2 of genotype B, except for one serotype aywl isolated from one occult infection. Conclusion The prevalence of overt and occult HBV infection is high among pregnant women in Anqing city of Anhui province.