以麝香百合‘白狐狸’(‘White Fox’)和‘雷山’(‘Lei Shan’)、东方百合‘水晶布兰卡’(‘Crystal Blanca’)和‘康斯坦撒’(‘Constansa’)的试管苗根尖为研究材料,用环己酰亚胺预处理,然后卡诺氏固定液固定,纤维素酶和果胶酶解离后采用涂片法制片,对4种观赏百合进行核型分析。结果表明,‘白狐狸’核型为3A,‘雷山’、‘水晶布兰卡’和‘康斯坦撒’的核型均为3B。核型公式分别为:‘白狐狸’2n=2x=24=4m+4st(2SAT)+16t,‘雷山’2n=2x=24=2m+2sm+10st(2SAT)+10t,‘水晶布兰卡’2n=2x=24=4m+10st+10t,‘康斯坦撒’2n=2x=24=4m(1SAT)+10st+10t。‘白狐狸’、‘雷山’、‘水晶布兰卡’及‘康斯坦撒’的核不对称系数分别为83.11%、82.78%、79.75%、81.22%。
Plantlet root tips of longiflorum hyhrids 'White Fox' and 'Lei Shan',oriental hybrids 'Crystal Blanca' and 'Constansa' were used as materials to analyze karyotype.The root tips were pretreated with cyclohexamide then fixed in Carnoy's solution and cell wall was digested with cellulase and pectinase.Finally,spreading method was used for preparing slides.The karyotype analysis result indicated that the karyotype of 'White Fox' was 3A,that of others were 3B.The karyotype formula was 2n=2x=24=4m+4st(2SAT) +16t for 'White Fox',2n=2x=24=2m+2sm+10st(2SAT) +10t for 'Lei Shan',2n=2x=24=4m+10st+10t for 'Crystal Blanca',2n=2x=24=4m(1SAT) +10st+10t for 'Constansa'.Karyotype asymmetry coefficient were 83.11%,82.78%,79.75%,81.22%,respectively.