利用SRAP标记对中国22个番木瓜主要栽培品种(系)进行亲缘关系和遗传多样性研究。结果表明:(1)筛选出的20对SRAP引物共扩增249条谱带,其中多态性条带llO条,多态性比率为43.20%,平均每对引物扩增的条带数和多态性条带数分别为12.45条和5.50条。(2)引物多态性信息含量(PIC)值变化范围为0.15~0.79,平均值为0.49。(3)聚类分析和主坐标分析显示,供试材料间的遗传相似系数为0.72~0.96,遗传多样性水平较低,在相似性系数为0.87时,可将所有试材分为3个类群。该研究结果有效地揭示了中国番木瓜主要栽培品种(系)资源的遗传背景和亲缘关系,可为番木瓜种质资源的分类、保护和有效利用以及新品种选育提供理论依据。
Genetic relationship and diversity of 22 papaya (Carica papaya L. ) major cultivars in China were analyzed based on sequence-related amplified polymorphism (SRAP). (1)The 20 pairs of SRAP primers generated a total of 249 fragments, and polymorphic bands were 110 which accounted for 43.20 % in the total amplified fragments. The number of amplified and polymorphic fragments was 12.45 and 5.50 per primer pair, respectively. (2)The polymorphism information content (PIC) values of these markers varied from 0.15 to 0. 79, averaging 0.49. (3)UPGMA cluster analysis and principal coordinates analysis (PCOA) showed that the range of genetic similarity (GS) was 0.72-0.96 among 22 papaya cultivars with a low genetic diversity. All cultivars could be divided into three groups at the GS of 0.87. These results indicated that SRAP analysis could be used to reveal effectively the genetic relationships of papaya cultivars in China,providing a scientific basis for the classification,protection and effective utilization of these resources and breeding new varieties.