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中文摘要：
[摘要]目的：研究酶解淫羊藿总黄酮制备宝藿苷I的工艺。方法：采用β-葡萄糖苷酶酶解淫羊藿总黄酮制备宝藿苷I，以宝藿苷T得率为指标，通过单因素考察pH值、温度、反应时间及酶与底物质量比对宝藿苷I得率的影响。结果：酶解反应的最适条件为温度50℃、pH5．0～5．5、反应时间7h、酶与底物质量比为1：20；以淫羊藿总黄酮计，宝藿苷I收率为9．25％。结论：β-葡萄糖苷酶酶解淫羊藿总黄酮制备宝藿苷I，得率高、工艺简便可靠、反应条件温和，适合工业化生产。
英文摘要：
OBJECTIVE To study the hydrolysis technology of Epimedium flavonoid by β-glucosidase for preparing baohuo side I. METHODS Taking the yield of baohuoside I as the index, β-glucosidase was used for enzymolysis of Epimedium fla vonoids and the effects of pH value, temperature,reaction time and the ratio of enzyme to substrate on yield of baohuoside I were studied. RESULTS The optimum enzymolysis with β-glucosidase was achieved at 50 ℃, a pH 5.0 - 5.5 Solution for7 h, and the ratio of enzyme to substrate is 1：20. The yield of baohuoside I was 9. 25% （Epimedium flavonoid was computed）. CONCLUSION It is feasible to prepare baohuoside I by enzymolysis with β-glucosidase, the condition is moderate and it is suitable for industrialization.
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