中文摘要：

为定量研究静态无血清悬浮培养的神经球内神经干/祖细胞的活性及代谢情况,取孕14 d的小鼠胚胎,从海马脑组织分离出神经干/祖细胞,培养过程中跟踪测定神经球直径的分布变化,利用CCK-8法测定活细胞数,同时对培养上清进行葡萄糖、乳酸、谷氨酰胺和氨的定量测定,并对相关细胞代谢动力学参数进行计算和分析.结果表明在培养过程中,神经球内细胞活性及细胞代谢均随着球直径的增加而减弱.在神经球平均直径为100μm左右,培养基中葡萄糖、谷氨酰胺浓度分别为36.38 mmol/L和1.33 mmol/L时,球心细胞生长开始受到抑制;而当神经球直径主体分布于100-150μm,培养基中葡萄糖浓度为31.11 mmol/L、谷氨酰胺浓度为1.15 mmol/L时,神经球内部细胞死亡数开始大于增殖数,同时细胞代谢降低到很低水平.表明随着神经球直径的增加而造成的传质困难对于其内部细胞的活性和代谢有重要影响.

英文摘要：

The aim of this study is to understand how the metabolism and viability neurospheres changed with the increase of neurospheres＇, diameters, the NSPCs of the cells in （neural stem progenitor cells ） were dissociated from the hippocampus tissue of embryonic 14 d （E14） mouse. During the culture, the diameter distribution of neurospheres and the cell viability were detected. Meanwhile, the concentrations of glucose, lactic acid, glutamine and ammonium in the media were measured, and the corresponding cellular metabolic rates were calculated and analyzed. The results show that the cell viability decreases with the increase of neur reaches about 100 Fm, and the concentrations of glucose and mmol/L, the growing of central diameter reaches about 100-150 OS g cells in neurospheres begins to pheres＇ diameters. When the diameter lutamine are 36.38 mmol/L and 1.33 be inhibited. Furthermore, when the μm, and the concentrations of mmol/L and 1.15 mmol/L, the number of dead cells inside neuros cells. And the metabolism of the cells declines limitation caused by the increase of neurospheresr metabolism gl ph ucose and glutamine are 31.11 eres exceeds that of proliferative to very low level. In conclusion, the mass transfer diameters has serious impact on the cell viability and metabolism