中文摘要：

以拟南芥野生型（Col）植株和突变体rfc3–1植株为材料,经叶片酶解、细胞裂解和细胞核收集与染色后,采用流式细胞仪检测分析了RFC3基因突变对叶片细胞数的影响,以建立植物细胞数直接高效检测试验体系。结果表明,野生型植株第1、2片真叶与第3、4片真叶的平均细胞数分别是rfc3–1植株相应叶片平均细胞数的1.6倍和2.6倍,说明RFC3基因突变严重影响了植物细胞的分裂增殖;建立了拟南芥叶片细胞数直接高效检测体系,其检测结果的变异值（CV）均小于5%。

英文摘要：

In order to establish a direct and high performance detection system for cell counts in leaves of Arabidopsis thaliana, wild type（Col） and replication factor C subunit 3 mutant（rfc3–1） plants were selected as materials to study the effects of gene mutation on cell counts by the approach of flow cytometry after enzyme digestion, cell lysis, nuclear collection and dyeing. The results showed that cell counts in the first and the second or the 3rd and the 4th of true leaf in wild type plants were 1.6 or 2.6 times that of rfc3-1 mutant plants respectively. It was indicated that proliferation of leaves was severely affected by gene mutation of RFC3.The established detection system for cell counts of leaves in Arabidopsis was direct and efficient with coefficient of variation（cv） less than 5%.