中文摘要：

目的：研究母爱剥夺是否诱发成年大鼠抑郁样行为表现,miR-16是否参与母爱剥夺诱发抑郁的病理过程。方法：在SD大鼠出生后第1天按窝随机分为母爱剥夺组（n=17）和正常对照组（n=17）。母爱剥夺组大鼠在出生后第1至14天每天接受6 h的母爱剥夺应激,正常对照组大鼠不接受任何实验处理。在大鼠13周龄时采用强迫游泳和糖水偏爱实验评定大鼠的抑郁水平;采用实时荧光定量RT-PCR检测大鼠海马miR-16的表达水平。结果：在强迫游泳实验中,母爱剥夺组大鼠的被动漂浮时间显著长于对照组大鼠,糖水偏爱率显著低于对照组（P〈0.05）;与对照组比较,母爱剥夺组大鼠海马内miR-16的表达水平显著增加（P〈0.05）,miR-16表达水平与抑郁样行为水平显著相关（P〈0.05）。结论：母爱剥夺能使大鼠成年后表现出抑郁样行为,海马内miR-16表达水平升高,miR-16可能参与母爱剥夺诱发大鼠抑郁样行为的病理过程。

英文摘要：

Objective： To detect the expression of miR-16 in the hippocampus of a rat depression model induced by maternal deprivation,and to explore whether miR-16 is involved in the pathological process of maternal deprivation-induced depression.Methods： Newborn SD rats were randomly divided into a maternal deprivation group（n=17） and a control group（n=17）.Rats in the maternal deprivation group experienced maternal deprivation for 6 h per day from 1st to 14th postnatal day,while rats in the control group rats received no treatment.When the rats were 13 weeks old,depression-like behaviors were assessed by forced swimming test and sucrose consumption test,and the expression of hippocampal miR-16 in rats was detected by real-time RT-PCR.Results： Maternal-deprived rats exhibited significantly longer passive floating time and lower sucrose preference rate than rats in the control group（P0.05）.Maternal-deprivation rats expressed higher level of miR-16 in the hippocampus than rats in the control group,and the expression level of miR-16 was significantly associated with the passive floating time（r=0.65,P0.05） and the sucrosepreference rate（r=–0.59,P0.05）.Conclusion： Maternal deprivation can induce depressive behaviors in rats and increase the expression of miR-16 in the hippocampus in rats.MiR-16 may be involved in the pathological mechanism of the maternal deprivation-induced depression.