目的:探讨白细胞介素19(interleukin-19,IL-19)及其受体(IL-20R1/IL-20R2)在不同类型慢性鼻-鼻窦炎[慢性鼻-鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSw NP)和慢性鼻-鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSs NP)]患者中表达的差异,并分析其与慢性鼻-鼻窦炎组织重塑的相关性。方法:研究共分3组:CRSw NP组(30例)、CRSs NP组(15例)和对照组(鼻中隔偏曲患者15例)。实时荧光定量PCR检测IL-19及其受体(IL-20R1/IL-20R2)和组织重塑因子基质金属蛋白酶(MMP)-2、MMP-9、金属蛋白酶组织抑制物(TIMP)-1的mRNA相对表达量;免疫组化检测IL-19及其受体的蛋白表达量;ELISA检测MMP-9、TIMP-1的蛋白表达量。结果:CRSw NP组IL-19、IL-20R1、IL-20R2和MMP-9的mRNA和蛋白表达量高于CRSs NP组和对照组(P〈0.05),CRSs NP组TIMP-1的mRNA和蛋白的表达量均高于CRSw NP组和对照组(P〈0.05);MMP-2的mRNA和蛋白的表达量在各组无显著差异。CRSw NP组IL-19、IL-20R1和IL-20R2均与MMP-9的mRNA相对表达量呈正相关(P〈0.05);IL-19及其受体与TIMP-1的mRNA相对表达量无明显相关。结论:IL-19及其受体的mRNA在CRSw NP中高表达,且与MMP-9 mRNA表达呈正相关,提示二者可能存在交互作用,可能参与慢性鼻-鼻窦炎的组织重塑。
AIM: To explore the differential expression of interleukin-19 (IL-19) and its receptors (IL-20R1/IL-20R2) in the samples of chronic rhinosinusitis (CRS) with or without nasal polyps (CRSwNP and CRSsNP), and to investigate the correlation of IL-19 and its receptors with tissue remodeling factors, including matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1 in CRS.METHODS: The polyps from CRSwNP patients (n=30), the sinus mucosa from CRSsNP patients (n=15) and the inferior turbinal mucosa of nasal septum de-viation patients (n=15) were collected. The expression of IL-19 and its receptors (IL-20R1/IL-20R2) in each group was detected by real-time RT-PCR and immunohistochemistry. The expression of tissue remodeling factors MMP-2, MMP-9 and TIMP-1 in different groups was detected by real-time RT-PCR and ELISA.RESULTS: Increased mRNA and protein expression levels of IL-19, IL-20R1, IL-20R2 and MMP-9 were observed in CRSwNP group compared with CRSsNP and control group (P〈0.05), while elevated expression level of TIMP-1 was observed in CRSsNP group compared with CRSwNP and control group (P〈0.05). The relative expression of MMP-2 among the 3 groups showed no obvious difference. The expression of IL-19 and its receptors showed significantly positive correlations with MMP-9 in CRSwNP (P〈0.05). No significant correlation between IL-19 and/or its receptors with TIMP-1 in CRSwNP group was observed.CONCLUSION: The high expression levels of IL-19, IL-20R1 and IL-20R2 and their positive correlations with MMP-9 in CRSwNP indica-te that IL-19 and its receptors may be involved in the tissue remodeling of chronic rhinosinusitis.