中文摘要：

目的研究CD133＋卵巢癌干细胞样细胞（ovariancancerstem-likecells，OCSLCs）向血管内皮细胞的分化。方法通过无血清培养方法诱导卵巢癌A2780细胞株来源的CD133＋OCSLCs；体外观察其在Matrigel基质胶上向血管内皮细胞分化的生物学行为，RT—PCR和Westernblot检测其成管后CD31的表达；通过裸鼠皮下移植瘤实验，免疫荧光法观察CD133＋OCSLCs在卵巢癌血管新生中的作用。结果成功诱导出CD133＋OCSLCs；体外成管实验结果显示CD133＋OCSLCs在Matrigel基质胶上具有成管能力，6h管腔样结构形成最为明显；免疫荧光、RT—PCR和Westernblot证实，OCSLCs成管后表达CD31内皮细胞标记物（P〈0．01）；CD133＋OCSLCs接种裸鼠皮下成瘤后，通过免疫荧光可观察到人源性肿瘤微血管的形成。结论CD133＋OCSLCs具有向血管内皮细胞分化的潜能。

英文摘要：

Objective To investigate the ability differentiating into vascular endothelial cells. Methods of CD133 ＋ ovarian cancer stem-like cells （OCSLCs） Ovarian cancer A2780 cells were cultured in serum- free medium to induce CD133 ＋ OCSLCs. Matrigel tube-forming assay was used to test the ability of CD133 ＋ OCSLCs differentiating into vascular endothelial cells, which was validated by CD31 expression with RT-PCR and Western blotting. The CD133 ＋ OCSLCs were subcutaneously injected to nude mice and then immuno- fluorescence staining of human specific endothelial markers was done to investigate the differentiation ability of CD133 ＋ OCSLCs in vivo. Results CD133 ＋ OCSLCs formed vessel-like tubes in Matrigel tube-forming assay, with the most obvious tube formation at 6 h. Immunofluorescence, RT-PCR and Western blot results showed that human specific CD31 was significantly up-regulated in Matrigel tube-forming assay （P 〈 0. 01 ）. Immuno- fluorescence staining of xenografts in nude mice showed the formation of human vessels, suggesting the ability of CD133 ＋ OCSLCs differentiating to tumor vascular endothelial cells in vivo. Conclusion CD133 ＋ OCSLCs have the potential of vascular endothelial cell differentiation, uncovering a novel mechanism of ovarian cancer angiogenesis and a novel way in anti-angiogenesis therapy of ovarian cancer.