类产碱假单胞菌(Pseudomonas pseudoalcaligenes)JS45能以硝基苯作为唯一碳源、氮源和能源生长。在本研究中,设置了硝基苯起始浓度分别为50、100、150、200、250和300 mg·L^-1。结果表明:菌株JS45虽然在降解高浓度硝基苯过程中出现迟滞现象,但仍可完全降解。盐度能抑制硝基苯的降解。JS45在温度30℃、pH 7.0~9.0时降解效果较好。硝基苯降解动力学符合修饰Gompertz模型,当硝基苯浓度升高时,模型参数迟滞时间(λ)逐渐增加,最大降解速率(Rm)先增大后减小;当硝基苯浓度为214.61 mg·L^-1时,λ和Rm分别为57.0 mg·(L·h)-1和4.31 h。
Pseudomonas pseudoalcaligenes JS45 has been shown to be able to utilize nitrobenzene as its sole source of carbon,nitrogen,and energy. The degradation kinetics of different initial concentrations of nitrobenzene( 50,100,150,200,250 and 300 mg·L^-1) were studied. The results showed that although a lag phase occurred when the nitrobenzene concentration was fairly high,strain JS45 could degrade nitrobenzene completely. The optimal degradation effect was achieved when temperature and pH were 30 ℃ and 7. 0-9. 0,respectively,but salinity inhibited the degradation process. The modified Gompertz model was successfully used to fit the degradation curve. With increasing initial nitrobenzene concentration,the lag phase( λ) became longer,and Rm first increased and then decreased. When the initial nitrobenzene concentration was 214. 61 mg·L^-1,the model parameters Rmand λ were 57. 0 mg·( L·h)- 1and 4. 31 h,respectively.