中文摘要：

目的：探讨盐酸苯肼注射浓度对小鼠血虚状态的影响,改良小鼠化学损伤性血虚证模型。方法：将ICR小鼠随机分为空白对照组（不进行造模）和4个试验组;试验组分别于第1、4、7天腹腔注射不同浓度的盐酸苯肼,检测造模前后小鼠体重、外周血红细胞数目和血红蛋白含量的变化,分析血虚状态持续时间。结果：腹腔注射盐酸苯肼可使ICR小鼠的外周血红细胞数量和血红蛋白含量显著降低（P〈0.01）,出现明显的中医血虚证表现。这一效应存在量效关系,但高浓度的盐酸苯肼影响小鼠存活率。结论：盐酸苯肼介导小鼠血虚证模型的最佳给药浓度为60 mg·kg-1体重。该模型的血虚状态至少可持续7 d,有利于进行相关科学研究。

英文摘要：

Objective ： To investigate the effects of phenylhydrazine hydrochloride on oxidative hemolysis in mice and establish a novel improved blood-deficient mouse model. Method： The ICR mice were randomly assigned to four experimental groups and a negative control group. The negative control group was given normal saline. The experimental groups were injected with different dose of phenylhydrazine hydrochloride at day 1,4 and 7, respectively. Changes of the indexes such as body weight（ BW ）, red blood cell count（ RBC ） and hemoglobin （ Hb ） were determined. Result ： After injecting phenylhydrazine hydrochloride, the number of RBC and hemoglobin were significantly decreased with dose dependance. However, mice given a higher concentration of phenylhydrazine hydrochloride treatment （ 120 mg~ kg-~ ） increased mortality. Conclusion ： The mouse model of blood-deficient by phenylhydrazine hydrochloride had been successfully established. The optimal dosing concentration was 60 mg ~ kg ~. Blood deficient syndrome could sustain at least 7 days, which was conducive to the relevant scientific research.