目的研究杜鹃兰Cremastra appendiculata假鳞茎的化学成分。方法利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备液相色谱等方法进行分离纯化,并通过1H-NMR、13C-NMR等波谱技术进行结构鉴定。结果从杜鹃兰假鳞茎90%乙醇提取物的醋酸乙酯部位中分离得到了11个化合物,分别鉴定为3,5,3′-三羟基联苄(1)、7-羟基-4-甲氧基菲-2-O-β-D-葡萄糖(2)、lignan glycoside(3)、ibotanolide A(4)、3,3′-二羟基-5-甲氧基-2,4-二(对羟基苄基)-联苄(5)、5,4′-二羟基-二苯乙基-3-O-β-D-葡萄糖苷(6)、3′-β-D-glucopyranosyloxy-4,5′-dihydroxy-3-methoxy-1,2-diphenylethane(7)、2,2′,7,7′-四羟基-4,4′-二甲氧基菲-1,1′-二菲(8)、7-羟基-2,4-二甲氧基菲(9)、β-胡萝卜苷(10)、对羟基苯甲醛(11)。结论化合物3~7为首次从该属植物中分离得到。
Objective To study the chemical constituents from the pseudobulbs of Cremastra appendiculata.Methods The compounds were isolated by repeated column chromatography with silica gel,Sephadex LH-20,and ODS-HPLC.The structures were elucidated by analysis of spectroscopic(1H-NMR and 13C-NMR) data.Results Eleven compounds were isolated from the Et OAc extract in the pseudobulbs of C.appendiculata.Their structures were identified as 3,5,3′-trihydroxybibenzyl(1),7-hydroxy-4-methoxy-phenanthrene-2-O-β-D-glucoside(2),lignan glycosides(3),ibotanolide A(4),3,3′-dihydroxy-5-methoxy-2,4-di(phydroxybenzyl) bibenzy(5),5,4′-bihydroxy-bibenzyl-3-O-β-D-glucoside(6),3′-β-D-glucopyranosyloxy-4,5′-dihydroxy-3-methoxy-1,2-diphenylethane(7),cirrhopetalanthin(8),7-hydroxy-2-4-dimethoxy-phenanthrene(9),β-daucosterin(10),and p-hydroxy benzaldehyde(11).Conclusion Compounds 3—7 are isolated from the pseudobulbs of C.appendiculata for the first time.