中文摘要：

通过冷冻干燥法进行壳聚糖（Chitosan）多孔支架材料的制各，使用Ⅰ型明胶对所制得的支架材料进行表面修饰，用于生物医用可降解复合材料的仿生构建。对所制各的支架材料分别进行密度、孔隙率和降解率等物理性质测定，并使用新西兰兔来源的骨髓间充质干细胞（Bone marrow mesenchymal stem cells，BMSCs）检验支架材料的细胞粘附和生物相容性能。以未经表面处理的该种材料作为对照组，对MSCs—Chitosan复合物分别进行扫描电镜和Hoechst染色等，检测MSCs（间充质干细胞）在支架材料表面的生长、粘附和分布形态。结果显示两种壳聚糖浓度制备的多孔支架材料的空隙率分别为（86．85±2．42）％和（71．50±3．29）％，孔径为90—130μm，MSCs在经明胶表面修饰的材料表面表现出良好的粘附、伸展性能。此外，本研究还对1-乙基-（3-二甲基氨基丙基）碳酰二亚胺（1-ethyl-（3-dimethylanimopropyl）carbodiimide，EDC）交联剂对壳聚糖基支架性能的影响进行了初步研究。研究表明，通过明胶修饰构建的仿生明胶／壳聚糖人工基质材料，具有良好的生物相容性能，作为一种理想的组织工程用支架材料，适于细胞在其上的粘附与增殖。

英文摘要：

The chitosan scaffolds were fabricated by lyophilization technique, and the prepared scaffolds were surface-modified by the gelatin to biomimetically construct a degradable composite for biomedical application. The density, porosity and degradation of the fabricated and modified scaffolds were determined; the cell adhesion behavior and biocompatibility of the treated scaffolds were detected by inoculation of the bone marrow mesenchymal stem ceils （BMSCs） derived from New Zealand rabbits, and the MSCs were inoculated onto the untreated scaffold too, which was used as the control sample group. The MSCs-chitosan composites were stained with Hoechst method and observed under fluorescence microscopes and scanning electron microscope （SEM） to study the cell growth, adhesion and distribution of the MSCs. The results show that the porosities of the scaffolds prepared with two different chitosan concentrations are （68.85±242）% and （71.50±3.29）%, respectively; the pore size is 90-130 μm, and the MSCs inoculated onto the treated surfaces present good adhesion and spreading ability. Besides, the effect of the crosslinking agent 1-ethyl- （3-dimethylanimopropyl） carbodiimide （EDC） on the performance of the scaffolds was initially investigated in this study. It can be concluded that the gelatin surface-modified biomimetic scaffolds have excellent biocompatibility and are appropriate for cell adhesion and proliferation. Therefore, it would be a promising scaffold for tissue engineering application.