中文摘要：

目的：探究相同频次、不同剂量雨蛙素腹腔注射诱导急性胰腺炎小鼠模型轻重程度的差异。方法：雨蛙素腹腔注射法诱导胰腺炎C57小鼠模型,共注射12次,每次间隔1 h,分别设置单次剂量为10μg/kg、50μg/kg、250μg/kg三个梯度。检测模型的血清淀粉酶、脂肪酶、胰腺组织水含量,以及胰腺组织HE染色、病理学评分和肺组织HE染色。结果：①雨蛙素造模组小鼠淀粉酶、脂肪酶和胰腺组织水含量均较正常组明显升高（P〈0.05）,50μg/kg剂量组各项数值达最大,250μg/kg剂量组无再明显升高（P〉0.05）。②造模组与对照组相比,胰腺组织和肺组织炎细胞浸润明显,低剂量（10μg/kg）注射胰腺组织呈水肿性改变,无出血坏死;常规剂量（50μg/kg）注射引起胰腺组织片状坏死出血;大剂量（250μg/kg）注射引起胰腺组织大范围坏死出血（P〈0.05）。结论：10μg/kg雨蛙素12次腹腔注射可诱发小鼠急性水肿性胰腺炎,50μg/kg雨蛙素12次腹腔注射能诱导出小鼠急性重症胰腺炎。

英文摘要：

Objective： To compare the characteristics of acute pancreatitis animal models induced by different doses of caerulein in common frequency. Methods： C57 mice were injected with caerulein for 12 times at 1 h interval via intraperitoneal pathway. The dose each time injected was divided into three groups （10 μg/kg, 50 p.g/kg and 250 μg/kg body weight） and each group was injected into six mice respectively. The amylase and lipase level, water content, HE staining were assessed. Results： ①The mice injected with eaerulein had higher enzyme levels and water content than control （P〈0.05）, and 50 μg/kg group had the highest level. ②Compared with control group, the mice injected with caerulein had more infiltration of inflammatory cells in the pancreas and lung tissues. The pan- creas in 10 μg/kg group only had manifestation of edema, but in50 μg/kg group it had laminar necrosis and in 250 μg/kg group it had wide range of necrosis （P〈0.05）. Conclusions： Low dose （10 μg/kg） caerulein can induce acute mild pancreatitis and common dose （50 μg/kg） can in- duce severe acute pancreatitis for 12 times injection in mice.