客观 Yersinia enterocolitica 是细胞外的病原体,它的相关抗原与主人免疫系统交往。我们在在 Y 的高度病原、糟糕病原的紧张之间的免疫学的特征调查了差别。enterocolitica。我们使用了 SDS 页并且描绘 lipopolysaccharide (LPS ) 的西方的弄污的方法, Yersinia 外部膜蛋白质(Yops ) ,膜蛋白质,和从糟糕病原的 Y 的整个房间的蛋白质。enterocolitica bio-serotype 2/O:9,从中国,和高度病原的 bio-serotype 1B/O:8 孤立,从日本孤立。这二 Y 拉紧的结果。enterocolitica 有不同 LPS 有免疫力的反应模式。他们的 Yops 的比较也显示出能在致病力说明了他们的差别的差别。膜和两紧张的整个房间的蛋白质是类似的;immunoblottting 证明 35 kD 并且也许 10 kD 蛋白质是在两紧张的 immunogens。得到主人免疫者反应的二紧张的主要抗原是 LPS 和膜蛋白质的结论由把蛋白质样品与参考作比较出现并且净化准备。
Objective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system. We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y. enterocolitico. Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide (LPS), Yersinio outer membrane proteins (Yops), membrane proteins, and whole-cell proteins from poorly pathogenic Y. enterocolitico bio-serotype 2/0:9, isolated from China, and highly pathogenic bio-serotype 1B/O:8, isolated from Japan. Results These two strains of Y. enterocolitica had different LPS immune response patterns. Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity. The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains. Conclusion The major antigens of the two strains e and membrane proteins, as shown by comparing preparations. citing the host immune protein samples with response were the LPS reference and purified