中文摘要：

目的：观察3’-大豆苷元磺酸钠对慢性心力衰竭大鼠心肌受磷蛋白（phospholamban，PLB）和Ca^2＋-ATP酶（Ca^2＋-ATPase，SERCA）mRNA表达的影响，探讨3’-大豆苷元磺酸钠抗心力衰竭的机制。方法：结扎大鼠冠状动脉前降支造成心肌梗死后慢性充血性心力衰竭（CHF）模型，采用实时荧光定量PCR技术观察。心肌PLB和SERCAmRNA表达以及3’-大豆苷元磺酸钠对其影响。结果：各给药组均可不同程度的下调PLBmRNA表达，上调降低SERCAmRNA表达，其中3’-大豆苷元磺酸钠0．4mg·kg。剂量组、地高辛组与模型组比较有显著性差异（P〈0．05）；各给药组均能降低PLB／SERCA比值，地高辛组和3’-大豆苷元磺酸钠0．4mg·kg^-1、0．2mg·kg^-1剂量组与模型组比较差异具有显著性（P〈0．05，P〈0．01）。结论：3’-大豆苷元磺酸钠治疗能提高SERCAmRNA表达，降低PLBmRNA表达，改善PLB／SER-CA比值，这可能是3’-大豆苷元磺酸钠抗心力衰竭的机制之一。

英文摘要：

Objective ：To observe the effect of 3＇-daidzein sulfonate sodium on the expression of PLB and SERCA mRNA in chronic heart failure（CHF） rats and the mechanism of 3＇-daidzein sulfonate sodium against heart failure. Methods：The model of CHF was induced by ligating the anterior descending branch of the left coronary artery. The rats were at random divided into six groups：sham operation group,model group,digaoxin group,three treatment groups treated with 0. 4mg · kg^-1 ,0. 2mg · kg^-1 and 0. 1 mg · kg^-1 dosage of 3＇- daidzein sulfonate sodium. After administration for 4 weeks. The effect of 3 ＇- daidzein sulfonate sodium in treatment on the expression of PLB and SERCA mRNA was observed by RT - PCR. Results ： All the treated groups could decrease the expression of PLB mRNA and increase the expression of SERCA mRNA in different degree, and there was significant difference between the 3＇-daidzein sulfonate sodium 0.4mg · kg^-1 dosage treated group, digaoxin group and model group （P 〈 0.05 ） ;All the treated groups could decrease ratio of PLB/SERCA. Compared with model group, digaoxin group and the 3＇- daidzein sulfonate sodium 0. 4mg · kg^-1,0. 2mg · kg^-1 dosage treated group had significant difference （P 〈 0. 05, P 〈 0.01 ）. Condusion：Glucoperiploeymar in treatment could increase the expression of SERCA mRNA, decrease the expression of PLB mRNA and improve the ratio of PLB/SERCA, and it might be one of the mechanism of 3＇- daidzein suffonate sodium in against heart failure.