中文摘要：

背景雌激素受体(嗯) 否定乳癌房间比嗯积极的房间更好攻击。cyclooxygenase-2 (COX-2 ) 和脉管的 endothelial 生长 factor-C (VEGF-C ) 的提高的层次表示在有教养的人的乳癌细胞被检测了并且与否定荷尔蒙受体地位被联系。在这研究，我们创造了嗯在 MDA-MB-231 房间到的稳定的 transfectants 探索效果嗯在房间生长和 COX-2 和 VEGF-C expression.Methods 上绿色荧光灯蛋白质(GFP )-ER plasmids 是稳定地 transfected 进嗯否定的 MDA-MB-231 房间。untransfected MDA-MB-231 房间， ER-transfected MDA-MB-231 房间和嗯积极的 MCF-7 房间的增长和迁居是坚定的。COX-2 的表达式，和 VEGF-C mRNA 和 VEGF-C 分泌物集中的层次是在这些房间 lines.Results 的 assayed ER-tranfected MDA-MB-231 房间的增长和移植能力显著地被减少(P < 0.05 ) 。COX-2 的表示比在 untranfected MDA-MB-231 房间在 ERa-tranfected MDA-MB-231 房间是显著地更低的。VEGF-C 的 mRNA 和蛋白质层次比在 untransfected MDA-MB-231 房间在 ERa-tranfected MDA-MB-231 房间是更低的(P < 0.05 ).Conclusions 嗯稳定的 transfection 禁止 MDA-MB-231 房间和 COX-2 和 VEGF-C 的减少表示的增长和迁居能力。增长和移植能力的减少可能与 COX-2 和 VEGF-C 表示的抑制有关。

英文摘要：

Background Estrogen receptor （ER）-negative breast cancer cells are more aggressive than ER-positive cells. Elevated levels of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor-C （VEGF-C） expression have been detected in cultured human breast cancer cells and are associated with negative hormone receptor status. In this study, we created ERα stable transfectants in MDA-MB-231 cells to explore the effect of ERα on cell growth and COX-2 and VEGF-C expression.Methods The green fluorescent protein （GFP）-ERα plasmids were stably transfected into ER-negative MDA-MB-231 cells. The proliferation and migration of untransfected MDA-MB-231 cells, ERα-transfected MDA-MB-231 cells and ER-positive MCF-7 cells were determined. The expression of COX-2, and the levels of VEGF-C mRNA and the VEGF-C secretion concentration were assayed in these cell lines.Results The proliferation and migration capacities of ERα-tranfected MDA-MB-231 cells were significantly decreased （P 〈0.05）. The expression of COX-2 was significantly lower in ERa-tranfected MDA-MB-231 cells than in untranfected MDA-MB-231 cells. The mRNA and protein levels of VEGF-C were lower in ERa-tranfected MDA-MB-231 cells than in untransfected MDA-MB-231 cells （P〈0.05）.Conclusions ERα stable transfection inhibits proliferation and migration capacities of MDA-MB-231 cells and decreases expression of COX-2 and VEGF-C. The decreases of proliferation and migration capacities may be related to suppression of COX-2 and VEGF-C expression.