中文摘要：

聚乳酸材料是一种应用广泛的可降解的组织工程支架材料，但是由于其表面亲水性差，影响了细胞的黏附和生长。为了提高聚乳酸材料表面的细胞相容性，首先利用溶液浇铸法制备聚L-乳酸（PLLA）膜材料，然后采用氨等离子技术进行表面处理，采用光电子能谱（XPS）和原子力显微镜（AFM）研究了处理条件对材料表面的化学结构和形貌的影响，结果表明处理后的PLLA膜的亲水性基团和表面平均粗糙度明显增加。最后研究了成骨细胞在材料表面的黏附，增殖和细胞周期的变化，结果表明成骨细胞在处理后的材料表面的黏附和生长较改性前有了很大提高，细胞能够更快地进入细胞分裂周期。

英文摘要：

Poly （ L-lactic acid） （PLLA） has been well documented for its excellent biodegradability, biocompatibility and nontoxicity properties and its degradation products arc biocompatible. However, poor hydrophilicity and the lack of natural recognition sites on the surface of PLLA for covalent cell-rccognition signal molecules to promote cell attachment. In this study, PLLA films were fabricated by solution casting method and subsequently their surface was treated by ammonia plasma. The changes of surface property and morphology were characterized by X-ray photoelectron spectroscopy （XPS） and atomic force microscopy （AFM）. The mouse osteoblasts were used to evaluate the cell compatibility of PLLA surfaces before and after treatment. The results showed that the hydrophilicity and surface roughness of PLLA films were improved after treatment. The adhesion and growth of mouse osteoblast on modified films were significantly improved. In conclusion, increased surface roughness and hydrophilicity of PLLA films could significantly enhance cell affinity that could be useful for applications of tissue engineering.