采集了从云南省16个县烟草主栽区的土壤样品129份.通过测定土壤抑制线虫生防菌Pochonia chlamydosporia孢子萌发率的大小将土样聚为抑菌率极显著差异的5个类群(P〉0.01).为研究抑菌土中的细菌种群,构建了强抑菌土样的细菌16S rRNA基因文库;利用限制性片段长度多态性(RFLP)对随机克隆进行筛选;测定代表克隆的16S rDNA序列;对强抑菌土中的细菌种群进行了系统发育分析,并表明Proteobacteria和Acidobacteria的细菌是强抑菌土中的主要细菌类群.
For the objective to study bacterial community related to soil fungistasis, 129 soil samples were collected from tobacco fields in 16 counties of Yunnan Province. After testing the fungistatic capability of inhibit conidialgermination of nematophagous fungus Pochonia chlamydosporia, these samples were combined into 5 significant groups (P 〉 0.01) according to their fungistatic values. The 16S rRNA gene library of strong fungistatic soil was constructed and random clones were screened by RFLP method. Phylogenetic analysis on bacterial community showed that members of Proteobacteria and Acidobacteria were dominant bacteria in fungistatic soil.