中文摘要：

本研究拟利用聚合酶链式反应-限制性片段长度多态性（PCR—RFLP）分析方法对赤拟谷盗Tribolium castaneum（Herbst）和杂拟谷盗Tribolium confusum（Jac du Val）进行分子鉴定，以期为仓储害虫管理和口岸检疫提供技术帮助和支持。采用通用引物对赤拟谷盗和杂拟谷盗的28SrRNA基因进行了PCR扩增、序列测定和分析，结果发现：扩增片段长约1070bp，该序列种内均无变异位点、种间有76个变异位点，即种内没有核苷酸替换发生、种间核苷酸替换发生76次，其中转换56次，颠换20次，转换／颠换的比值为2．80。用限制性内切酶PvuI对赤拟谷盗和杂拟谷盗的28S rRNA基因扩增产物进行酶切，电泳检测显示，赤拟谷盗和杂拟谷盗的28S rRNA基因扩增产物的PvuI酶切图谱（分别产生2个和3个酶切条带）明显不同，因此本研究建立的28S rRNA基因PCR—RFLP方法可用于赤拟谷盗与杂拟谷盗的分子鉴定。

英文摘要：

The red flour beetle, Tribolium castaneum （Herbst）, and the confused flour beetle, Tribolium confusum （Jacquelin du Val）, are two morphologically similar, economically important, and widely distributed stored - product pests throughout the world. To improve the level of pest management and port quarantine, it is important to identify and control them. A molecular diagnostic method using polymerase chain reaction -restriction fragment length polymorphism （PCR -RFLP） was developed in order to distinguish Tribolium flour beetles, based on partial 28S rRNA gene sequence. In the sequence, 76 interspecific variations were located, whereas no intraspecific variation was found in both beetle species. The nucleotide substitutions between 28S rRNA gene sequences of Tribolium beetle species consisted of 56 transitions and 20 transversions, and the transition/transversion （ti/tv） ratio was 2. 80. Restriction digestion analysis of the unpurified PCR products of Tribolium 28S rRNA gene, using PvuI endonuclease, generated reproducible species -specific restriction patterns showing two fragments for T. castaneum and three fragments for T. confusum. The PCR - RFLP assay developed in this study proved to be an accurate and reliable method for identifying Tribolium flour beetles.