中文摘要：

目的 构建并鉴定细粒棘球绦虫转基因植物载体重组pBI-Eg95-EgA31质粒。方法 从细粒棘球蚴包囊中分离原头节,超声粉碎后抽提总RNA为模板,采用RT-PCR方法 分别扩增Eg95和EgA31编码基因,然后采用基因拼接法（gene SOEing）扩增Eg95-EgA31融合基因;将该融合基因定向克隆到植物表达载体pBI121中构建pBI-Eg95-EgA31重组质粒;电穿孔转化根癌农杆菌（Agrobacterium tumefaciens,At）LBA4404株,抽提质粒进行酶切及PCR鉴定。结果 电泳及测序证实1 016bpEg95-EgA31融合基因克隆成功。经酶切及PCR证实重组质粒pBI-Eg95-EgA31成功转入根癌农杆菌。结论 成功构建了细粒棘球绦虫转基因植物载体重组pBI-Eg95-EgA31质粒,为进一步构建细粒棘球绦虫转基因植物疫苗奠定了基础。

英文摘要：

To construct and identify the transgenic plant vector for recombinant plasmid pBI-Eg95-EgA31 of Echinococcus granulosus, the total RNA was extracted from hydatid cyst pro-toscoleces shattered by supersound, and the Eg95 and EgA31-coding genes were amplified by RT-PCR. The Eg95-EgA31-fusion gene was obtained with GOEing. and then the fusion gene was cloned into the plant expression vector pBI-121 to construct the recombinant plasmid pBI-Eg95-EgA31. As demonstrated by electrophoresis and sequencing, the Eg95-EgA31 fusion gene with 1016 bps was successfully amplified and the recombinant plasmid pEgBI-Eg95-EgA31 was also successivelly constructed, as confirmed by restriction endonuclease digestion and PCR. The results in the present investigation would provide a basis for the further study on the trangcnic plant vaccine for E granulosus infection.