中文摘要：

参照GenBank中牛整合素α4基因序列,合成了牛整合素α4亚基753bp（192～944nt）的cDNA片段,克隆至原核表达载体pET-28a（＋）中,转化入BL21,IPTG诱导表达。表达产物经Western blot鉴定,结果表明成功表达了相对分子质量约为30 000的α4蛋白。超声破碎细菌后,目的蛋白主要以包涵体形式存在。蛋白纯化后进一步复性,免疫大鼠获得多抗,间接ELISA检测多克隆抗体效价为1∶32 000。这为进一步研究牛整合素α4β7的功能奠定了基础。

英文摘要：

According to the bovine integrin α4 nucleotide sequence reported in the GenBank,the fragment of α4 subunit was synthetized,which was 753 bp.It was cloned into pET-28a（＋） to construct the recombinant plasmid,then transform into BL21.After being induced by IPTG,the fragment of α4 was expressed in BL21.The expression product was identified by Western blot.Results showed that the fragment of α4 with relative molecular mass of 30 000 was expressed successfully.The expression product was mainly in the form of inclusion body.After purification,the target protein was renaturated for immuning rat.The polyclonal antibody titer of rat anti-α4 was 1：32 000 by indirect ELISA.The results of this study lay a foundation for further study on the function of bovine integrin α4β7.