[目的]建立印楝中nimbin的高效液相色谱定量分析方法。【方法】采用0Ds色谱柱,流动相为甲醇一水(体积比为70:30),流速1mL/min,检测波长UV217nm。【结果】方法线性相关系数为0.99994(0.0940-9.3954ng),相对标准偏差为0.14%,回收率为94.9%。【结论】方法简便、重现性好。
[Aims] It was to establish a quantitative analysis method of nimbin in neem by high-performance liquid chromatography. [Methods] A ODS column was used. The mobile phase was the mixture of methanol-water (70 : 30, by vol). Flow rate was 1.0 mL/min and wavelenth of UV detector was 217 nm. [Results] The linear correlation coefficient was 0.99994 (0.0940-9.3954 μg), relative standard deviation(RSD) was 0.14% and recovery was 94,9%. [Conclusions] This method is convenient with good reappearance.