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中文摘要：
在已构建高频再生体系的基础上，研究构建美洲商陆遗传转化体系的条件。结果表明：250mg／L头孢霉素既能有效抑制根癌农杆菌的增殖，且对不定芽分化影响相对较小；美洲商陆对潮霉素高度敏感。美洲商陆的遗传转化过程为：无菌苗上部经增粗培养后，取茎节在不定芽分化培养基（ABDM）中预培养5d，在D600nm为0．6的菌液中侵染3～5min，再转接到ABDM中黑暗、26℃共培养3d；然后外植体经冲洗后转接至含250mg／L头孢霉素的ABDM中进行脱菌、分化以及不定芽伸长培养；待不定芽长至2cm以上时，截取并转接到含3mg／L潮霉素的生根培养基中进行抗性芽筛选，筛选出的抗潮霉素植株再经GFP显色、PCR、分子杂交等方法进一步阳性确认。
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