中文摘要：

应用Real-time RT-PCR检测了水稻条纹病毒（Rice stripe virus，RSV）在2种水稻品种（品系）武育粳3号和KT95-418的悬浮细胞内复制变化和相对含量的差异，结合传统生物学接种试验，确定了这2个品种（品系）对RSV抗性的差异．结果表明，RSV在武育粳3号的悬浮细胞内24h达到复制高峰，病毒含量为侵染初期的7．46倍．而在KT95-418的悬浮细胞内，RSV达到复制高峰需要36h，病毒含量为侵染初期的4．51倍．利用病毒生物学接种的方法，武育粳3号发病率达91．7％，而KT95-418仅为36．0％．由此可见，KT95-418较武育粳3号对RSV具有较高的抗病性．因此，Real-time RT-PCR方法与传统生物学接种试验方法相比，具有更高的准确性和灵敏性，可以作为传统品种抗病性鉴定的验证手段．

英文摘要：

The different resistance against Rice stripe virus （RSV） between rice Wuyujing 3 and KT95- 418 was investigated by detecting the replication cycle and relative amount of RSV in two varieties rice suspension cells by using Real-time RT-PCR assay and conventional RSV infected assay. The results showed that it needed 24 and 36 h when RSV replication reached its peak in rice suspension ceils of Wuyujing 3 and KT95-418 respectively, and at their peaks, the amount of RSV increased 7.46 and 4. 51 times respectively. The infected incidences of Wuyujing 3 and KT95-418 were 91.7% and 36. 0% by conventional RSV infected method. It was showed that KT95-418 had higher resistance against RSV than Wuyujing 3. In comparison with conventional RSV infected methods, Real-time RT-PCR assay was more accurate and sensitive, and would be used to validate the result of conventional RSV infected assay.