中文摘要：

以川芎咖啡酸-3-O-甲基转移酶基因（LCCOMT）为表达对象,将含有6＊His标签的LCCOMT基因连接pET28a表达载体后转化大肠杆菌BL21,采用原核表达的方法,优化了LCCOMT基因的表达条件。结果表明：当诱导时间为4h,IPTG终浓度为2.0mmol·L^-1,诱导温度为37℃时,重组LCCOMT蛋白的表达量达到最大。溶解性检测结果表明,LCCOMT重组蛋白以可溶性蛋白的形式存在,占总蛋白的9%,该试验可为LCCOMT的大规模表达纯化奠定基础。

英文摘要：

In order to efficiently express Ligusticum chuanxiongcaffeic acid 3-O-methyltransferase（LCCOMT）in E.coli,pET28a-LCCOMT expression vector,which contained LCCOMTgene with 6＊His labels,was transformed into E.coli BL21.The expression conditions of LCCOMTgene were optimized.The results showed that when the induction time was4 hours,IPTG concentration was 2.0mmol·L^-1 and temperature was 37 ℃,the expression of LCCOMT recombinant protein might reach the maximum.Furthermore,solubility test results showed that the LCCOMT recombinant protein existed in the form of soluble protein.Therefore,this research laid a foundation for the future large-scale expression and purification of LCCOMT protein.