中文摘要：

目的：研究顶坛花椒的抗氧化活性成分。方法：采用1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除法筛选测定顶坛花椒果皮的甲醇提取物及其不同极性溶剂萃取部位抗氧化活性,筛选出抗氧化活性部位,然后采用柱层析法及高效液相色谱法分离活性部位,通过13C-NMR、1H-NMR和质谱法,结合文献数据鉴定化合物结构。结果：顶坛花椒的甲醇提取物具有显著DPPH自由基清除能力,其乙酸乙酯萃取部位为活性部位,从中分离得到的5个主要成分分别被鉴定为：2’-羟基-β-山椒素（1）,异鼠李素-3-O-β-半乳糖苷（2）,槲皮素-3-O-β-半乳糖苷（3）,槲皮素-3-O-β-葡萄糖苷（4）,槲皮素-3-O-β-（4’’-O-硬脂酰基）葡萄糖苷（5）,其中（5）为首次从花椒属植物分离得到。结论：顶坛花椒果皮具有显著的抗氧化作用,主要活性成分为其中的酰胺类和黄酮类组分。

英文摘要：

Objective： To investigate the antioxidant constituents from Z. planispinum var. Dingtanensis. Methods： DPPH method was used to evaluate the antioxidant activity, and combined chromatographic techniques were used to isolate and purify the compounds, NMR and MS methods were used to determine structures, with the comparison of reported data. Results： the Et OAc fraction of the methanol extracts was the most active fraction, and five compounds（1~5） were isolated from it. The structures of compounds were identified as 2＇-hydroxy-b-sanshool（1）, isorhamnetin-3-O-β-pyranogalactoside（2）, quercetin-3-O-β-pyranogalactoside（3）, Quercetin-3-O-β-pyranoglucoside（4）, quercetin-3-O-β-（3＇＇-Ostearoyl）-pyranoglucoside（5）. Conclusion： The antioxidant activity of Z. Planispinum var. dingtanensis was owed to the flavonoid and amide constituents.