中文摘要：

油体钙蛋白不仅参与了油体的形成，而且可能与植物耐受干旱胁迫有关．目前对水稻中的油体钙蛋白的功能了解很少．本文通过对水稻基因组数据库的序列搜索，确定了水稻油体钙蛋白基因家族的6个成员．通过对这些基因的序列、结构和染色体位置分析，表明水稻油体钙蛋白基因家族的扩增可能与片段复制和串联重复有关．通过对这些基因与其他物种中的油体钙蛋白基因的系统进化分析及功能比较分析，揭示了拟南芥和水稻中功能可能对应的成员．为了确定与干旱胁迫响应相关的油体钙蛋白基因，通过荧光定量PCR检测了水稻油体钙蛋白家族在根、叶、花中的表达，以及在这些组织中响应干旱胁迫的表达情况，发现有3个基因成员在不同组织中受干旱胁迫的诱导，为进一步对它们展开功能分析奠定了基础．

英文摘要：

Caleosins are proteins that are components of plant oil bodies, and may be involved in the drought tolerance of plants. So far, the rice caleosin gene family remains largely uncharacterized. Using BLAST analyses against the rice genome database and other bioinformatic tools, 6 members of the caleosin family were identified in the rice genome. The evolutionary relationships among caleosin genes from rice, Arabidopsis and other species were analyzed. In addition, fluorescent real-time PCR method was employed to analyze the expression patterns of the caleosin gene family in different organs such as root, leaf, and flower, and to examine their expression-level changes in response to drought or osmotic stress. The results suggested that some members of the caleosin gene family are involved in drought tolerance, thus providing important background information for further study on their physiological roles under drought stress.