陆地棉双隐性核雄性不育系ms5ms6。已有较大的应用规模,但雄蕊败育的结构基础尚不明确。以核雄性不育系ms5ms6。为材料,利用透射电镜对四分体时期和刚长出小刺突的小孢子时期的花药进行超微结构观察。发现在小孢子时期,败育花药小孢子只有外壁内层,可育小孢子此时已具有外壁外层和外壁内层了。在整个不育花药的发育过程中,不管是在小孢子还是绒毡层细胞中,内质网都异常,脂肪的积累少,这可能是导致小孢子败育最重要的原因。
The double recessive nuclear male-sterile line mssms6 of upland cotton ( Gossypium hirsutum L. ) has been widely used in a large-scale. However, the structural basis of anther abortion in cytology of this material is still unknown. Transmission electron microscopy was used to observe nuclear male-sterile line ms, ms6 in stages of tetrads and microspores on which there are small spinescent protuberances. The results show that the male-sterile microspores have only endexine during the stage of microspore, however, the fertile microspores have sexine and endexine of cell walls. In the microspores and tapetum ceils, the abnormal endoplasmic reticulum and no fat accumulation were found during male sterile anther development, which might be the most important reason of microspores abortion.