采用开顶式气室(open top chambers,OTCs)装置,以水稻品种“3694繁”(Oryzasativa L.)为材料,研究3种处理:过滤大气(CF,O3浓度约为20nl.L-1)、环境大气(NF,O3浓度约为40nl.L-1)和高浓度O3(EO,O3浓度约为75nl.L-1)下叶片可溶性蛋白质含量、膜脂过氧化程度与主要保护酶活性的变化.结果表明:过滤大气与环境大气处理之间各个指标无显著差异.与CF处理相比,高浓度O3处理条件下水稻叶片中可溶性蛋白含量显著下降,而过氧化氢(H2O2)和抗坏血酸(ASA)含量显著增加;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性升高,最大升高幅度分别为93.7%、39.9%和312.4%;抗坏血酸过氧化物酶(APX)活性呈先升高后下降趋势;不同O3处理条件下叶片中丙二醛(MDA)含量无显著变化.表明抗氧化系统可有效抑制水稻叶片中的O3胁迫引起的膜质过氧化作用,说明水稻品种“3694繁”对O3胁迫有一定抗性.
With rice ( Oryza sativa L. ) cultivar 3694 Fan as test material, and by using open top chambers, the effects of different ozone (O3 ) concentration on the leaf soluble protein content, lipid peroxidation, and antioxidant system were applied. Three treatments were applied, i. e. , charcoal-filtered air (CF, 20 nl 03·L^-1), non-filtered ambient air (NF, 40 nl 03· L^-1), and ele- vated 03 ( EO, 75 nl 03· L^-1 ). The results showed that there were no significant differences in the test variables between treatments CF and NF. Compared with CF, EO induced a significant decrease of soluble protein content and significant increases of H2 O2 and ASA contents and SOD, CAT and POD activities. In treatment EO, the SOD, CAT and POD activities increased by 93.7% 39. 9% and 312. 4%, respectively, as compared with treatment CF, while the APX activity tended to increase at first and decrease then. MDA content had no significant differences among the treat- ments, indicating that leaf antioxidant system could significantly prevent the lipid peroxidation induced by elevated O3, and rice cultivar 3694 Fan could resist O3 to some extent.