中文摘要：

目的比较不同比例激活剂对富血小板凝胶的生物学影响，研究其用于角膜基质再生的可行性。方法抽取兔全血，经二次离心法制备富血小板血浆（PRP），将PRP与激活剂按不同比例激活，制备PRP凝胶。实验分为3个实验组，分别为9∶1组（PRP∶激活剂=9∶1）、11∶1组（PRP∶激活剂=11∶1）及5∶1组（PRP∶激活剂=5∶1）。将兔角膜基质细胞与PRP凝胶共同培养，进行组织形态学观察，用细胞增殖-毒性检测试剂盒法，分别检测3个实验组PRP凝胶释放的复合生长因子促角膜基质细胞的增殖作用。对不同实验组制备的PRP凝胶与双层猪角膜基质进行黏附性观察。结果9∶1组制备的PRP凝胶促进兔角膜基质细胞的增殖和活化作用强于其他实验组。3个实验组中，只有9∶1组制备的PRP凝胶苏木精-伊红染色法染色结果显示，呈有序的网状支架结构，能紧密黏附并固定双层猪角膜基质。结论PRP与激活剂以9∶1比例制备的PRP凝胶促角膜基质细胞增殖作用最强，同时具有有序网状结构和良好的组织黏附性及相容性，是一种可以用于角膜基质再生的良好支架。

英文摘要：

Objective To compare the effect of different proportions of activators on the biological functionof platelet-rich gel and to reaserch the feasibility for the reconstruction of corneal stroma.Methods Rabbitblood was extracted and platelet-rich plasma(PRP)was prepared by two-step centrifugation.Platelet-rich gelswere prepared by activation of PRP with thrombin activators in different proportions and divided into threegroups.There were group9:1(PRP:activators=9:1),group11:1(PRP:activators=11:1)and group5:1(PRP:activators=5:1).The rabbit keratocytes were seperately co-cultured with the three platelet-rich gelsand the morphological features were observed.CCK-8assay was used to analyze the influences of compositegrowth factors released from the platelet-rich gel on the proliferation of rabbit keratocytes in the three groups.The adhesive properties of the three platelet-rich gels were evaluated after adhesion to the double-layerporcine corneal stroma.Results The platelet-rich gel of the group9:1had better effect on promoting cellproliferation than those of other two groups.HE staining displayed that only the platelet-rich gel prepared inthe group9:1formed an orderly network scaffold that could closely attach to the double-layer porcine cornealstroma.Conclusions The platelet-rich gel,prepared by combining PRP with activators in9:1proportion,can effectively promote the proliferation of keratocytes,and has the nature of orderly network structure,effectiveadhesiveness and favorable biocompatibility.These properties indicate that the platelet-rich gel prepared bycombining PRP with activators in9:1proportion is an excellent scaffold for the reconstruction of cornealstroma.