中文摘要：

利用高效毛细管法（HPCE）对人工倾斜的中林46杨（Populus×euramericana（Dode）Guiner CL.Zhonglin-46）树干形成层内源激素进行测定。结合自身试验材料的特殊性,优化样品前处理过程,选择符合自身材料的最佳电泳条件,探索出测定树干形成层5种内源激素赤霉素（GA3）、吲哚-3-乙酸（IAA）、吲哚-3-丁酸（IBA）、玉米素（Z）和脱落酸（ABA）的方法。最佳条件为：电压16 kV、柱温20℃、缓冲液pH 9.25、SDS浓度50mmo.lL-1。5种内源激素的相关系数R2为0.978 6~0.999 9,回收率为93%~105%,变异系数≤3.93%。

英文摘要：

The endogenous hormones in inclined stem cambium of poplar（Populus×euramericana（Dode） Guiner CL.Zhonglin-46） were determined by high-performance capillary electricity（HPCE）.The five endogeneous hormones such as gibberellic acid（GA3）,indole-3-Acetic acid（IAA）,indole-3-butyric acid（IBA）,zeatin（Z） and abscisic acid（ABA） in cambium of poplar stem were separated by optimizing the pre-processing procedure and the electrophoretic conditions suitable for the particularity of the material.The best conditions are as follows：voltage 16 kV,column temperature 20℃,the pH 9.25 for electrolyte solution,and concentration of SDS is 50 mmol·L-1.The corresponding coefficient of five kinds of endogenous hormones reach from 0.9786 to 0.9999.The recoveries of hormones vary from 93% to 105%,and the coefficients of variation are less than 3.93%.