中文摘要：

目的 探讨一种稳定的多次乌拉坦注射诱导小鼠肺癌模型的构建方法，比较BALB/C及C57BL/6J小鼠对该肺癌模型的敏感性。方法 10只BALB/C小鼠及10只C57BL/6J小鼠适应性饲养3周， 随后每只小鼠分别被给予每周1次腹腔注射1 g/kg体重乌拉坦，连续注射10周，继续喂养15周后处死小鼠取肺组织。由3名不同的检测者在解剖显微镜下观察计数肺组织表面肿瘤数目并以直径记录肿瘤大小；HE染色检测肺组织病理变化。结果 多次乌拉坦注射诱导的BALB/C及C57BL/6J小鼠肺癌发生率均为10/10（100%）；BALB/C小鼠荷瘤数明显多于C57BL/6J小鼠（P〈0.01），同时，直径也大于C57BL/6J小鼠（P〈0.05）；HE染色显示多次乌拉坦注射诱导的肺癌有非典型性腺瘤增生及腺瘤两种病变类型。 结论 BALB/C和C57BL/6J小鼠均可以作为多次注射乌拉坦诱导性肺癌模型的动物，BALB/C小鼠对该肺癌模型的敏感性高于C57BL/6J小鼠。

英文摘要：

Objective To explore a method for establishing stable mouse model of lung cancer, and compare the susceptibility of BALB/C and C57BL/6J mice for multiple urethane injections-induced lung carcinogenesis. Methods Ten BALB/C and ten C57BL/6J mice were normally feed for 3 weeks, and then received 1 g/kg urethane by weekly intraperitoneal injection for 10 weeks, followed by 15 weeks’ latency period. Finally, all the mice were sacrificed and the lung tissues were isolated. During the autopsy procedure, the tumor lesions on lung surfaces were carefully counted by three blinded readers, and the lesion diameter was measured with the aid of digital calipers under a dissecting microscope; HE staining was used to examine the pathological characteristics of lung tissues. Results The results showed that the incidences of both BALB/C and C57BL/6J mice were 10/10（100%） ; The number of BALB/C mice-bearing lung tumors was significantly more than that of C57BL/6J mice （P〈0.01）, meanwhile the diameter of BALB/C mice-bearing tumor was larger than that of C57BL/6J mice （P〈0.05）; The results of HE staining showed that atypical adenomatous hyperplasia and adenomas were the two main pathological types of multiple urethane injections-induced lung cancer model in this study. Conclusion Both BALB/C and C57BL/6J mice could be used as model animals of multiple urethane injections-induced lung cancer, and the susceptibility of BALB/C mice was higher than that of C57BL/6J mice.