中文摘要：

目的 寻找有效的跨圆窗膜载药体,制备多孔明胶微球结合壳聚糖/β-GP溶液的温敏特点,制备新型可注射、可降解、无毒副作用并具有缓释效应的药物载体,为后续动物实验提供研究基础。方法 利用化学反应合成碳酸钙（CaCO_3）模板,采用W/O型乳化-固化法制备了明胶-碳酸钙复合微球,用EDTA处理除去碳酸钙,得到多孔的明胶微球。并以环境扫描电子显微镜对多孔明胶微球进行表征。利用壳聚糖与β-GP合成溶液物理混合形成壳聚糖多孔明胶微球温敏凝胶载药体。结果 扫描电镜显示,明胶微球表面呈多孔结构,粒径在2~4um之间。壳聚糖/β-GP冻干后呈多孔网络状。壳聚糖/β-GP具有温敏性,且两者共混后温敏性不变。结论 壳聚糖/β-GP溶液共混可制备成具有温敏效果的凝胶载药体,为跨膜药物转运的研究提供基础。

英文摘要：

Objective To search for an effective drug carrier to penetrate the round window membrane, the porous gelatin microsphere combined the thermo-sensitive chitosan/13 -GP gelatin was prepared. Methods CaCO3 template was synthesized chemically, and gelatin CaCO3 microsphere was prepared by W/O emulsive solidified method. Then CaCO3 was removed by EDTA, and porous gelatin microsphere was prepared. Scanning electron microscope was utilized for microsphere characterization. Thermo-sensitive drug carrier of chitosan porous gelatin mierosphere was obtained by physical mixation of chitosan and β -GP. Results The chitosan/β -GP solution was injectable, the diameters of chitosan/β -GP gelatin microspheres were about 2 - 4him with porous network after freezing and thermo sensitivity. Condusion Thermo-sensitive drug carrier of chitosan/β -GP gelatin microspheres would be useful for transmembrane drug delivery.