中文摘要：

本研究旨在分析miR-155在长白猪（瘦肉型）和通城猪（脂肪型）骨骼肌发育过程中的表达变化,以了解其在猪肌肉发育中的重要作用。选用长白猪和通城猪不同发育时期（胚胎期12个时期,出生后10个时期）骨骼肌为材料,利用Stem-Loop RT-PCR方法检测miR-155的表达变化。此外,本研究还分析了miR-155在成年通城猪各组织的表达。结果,miR-155在长白和通城猪骨骼肌发育过程中差异表达呈现不同的表达模式。在通城猪中,miR-155在小肠和肺中表达量较高,在心脏和胎盘中度表达,而在其他组织中表达较低。结果提示,miR-155可能参与猪肌细胞增殖和分化,从而影响猪骨骼肌发育,与猪肌肉的表型有关。

英文摘要：

The aim of this study was to detect the expression of miR-155 in skeletal muscle development in Landrace（lean-type） and Tongcheng（obese-type） pigs,and explore the roles of miR-155 in muscle development.Based on the skeletal muscle tissues of 22 stages（12 prenatal and 10 postnatal stages） in Landrace and Tongcheng pigs,respectively,stem-loop RT-PCR was performed to detect the miR-155 expression.Furthermore,the expression of miR-155 in various tissues of adult Tongcheng pigs was detected.The miR-155 differential expression in skeletal muscle development exhibited different expression patterns between Landrace and Tongcheng pigs.In adult Tongcheng pigs,miR-155 was abundantly expressed in small intestines and lung,moderately in heart and placenta,and weakly in other collected tissues.The result indicate that miR-155 may be involved in myofibre proliferation and differentiation and further contribute to skeletal muscle development and muscle phenotype with different genotypes.