中文摘要：

目的探讨多克隆神经细胞粘附分子抗体（P-NCAM-Ab）对A型肉毒毒素（BTX—A）生物学效应的影响。方法选取雄性SD大鼠90只，按随机数字表法将其分为正常对照组、BTX—A组、P—NCAM—Ab组，每组30只。给予BTX—A组和P—NCAM—Ab组大鼠右侧腓肠肌BTX—A0．5u（100μ1）注射，正常对照组大鼠则注射同等体积的生理盐水，第3天时在P—NCAM—Ab组大鼠右侧腓肠肌同一位点注射P—NCAM—Ab20U。注射前、后，动态测量大鼠腓肠肌肌肉的收缩强度及湿重变化，并通过乙酰胆碱酯酶染色和氯化金染色观察大鼠运动终板及可视神经纤维的变化情况。结果干预后，正常对照组大鼠腓肠肌肌肉的收缩强度随时间延长而逐渐增加，BTX—A组和P—NCAM—Ab组则呈现出先下降后上升的趋势。与正常对照组同时间点比较，BTX—A组和P—NCAM—Ab组大鼠注射BTX—A后3d及1，2，4，6，8，10周时的腓肠肌肌肉收缩强度均较弱（P〈0．05）；与BTX．A组同时间点比较，除注射后3d及1周，P—NCAM—Ab组大鼠剩余时间点腓肠肌肌肉的收缩强度均较BTX—A组弱（P〈0．05）。BTX—A组和P—NCAM—Ab组大鼠腓肠肌收缩强度的恢复时间长于正常对照组（P〈0．05），且P—NCAM—Ab组的恢复时间长于BTX—A组（P〈0．05）。BTX—A组和P—NCAM—Ab组大鼠腓肠肌湿重百分比随时间增长呈现出先下降后上升的趋势，且注射后3d及1，2，4周，BTX—A组和P—NCAM—Ab组组间比较，差异有统计学意义（P〈0．05）。注射BTX—A后1，2，4，8，12周，BTX—A组及P—NCAM—Ab组运动终板染色均逐渐加深，但P—NCAM—Ab组大鼠运动终板同时间点内染色均浅于BTX—A组。BTX—A组和P—NCAM—Ab组大鼠运动终板阳性反应区域平均光密度值随时间延长而逐渐增加，且P—NCAM—Ab组在第1，2，4，8，12周时的平均光密度值低于BTX—A组（P〈0．05）。BTX—A组和P．NCAM?

英文摘要：

Objective To investigate the impact of polyclonal neural cell adhesion molecule antibody （P-NCAM-Ab） on the potency of botulinum toxin A （BTX-A）. Methods Ninety male Spragne-Dawley rats were randomly divided into 3 equal groups： a normal control group, a BTX-A group and a P-NCAM-Ab group. The rats in the normal control group were injected with 100 μl of saline solution in their right gastrocnemius, while those in the BTX-A and P-NCAM-Ab groups were injected with 100 p.1 of BTX-A （0. 5 U）. In addition, the rats in the P-NCAM-Ab group were also injected with 100 μl of P-NCAM-Ab （the dosage was 20 U） at the same site on the 3rd day after the BTX-A injection. The rats＇ gastrocnemius muscle strength was evaluated with a self-made system for e- valuating neuromuscular function before and after the toxin injection, on the 3rd day, as well as 1 , 2, 4, 6, 8, 10 and 12 weeks after the BTX-A injection. Any wet weight changes in the muscles were observed, and immunochemis- try methods were employed to observe any structural changes in the motor endplates and nerve fibers at the different time points. Results After the saline injection, the average gastrocnemius muscle strength of the control group in-creased with time, while strength in lhe BTX-A and P-NCAM-Ah groups demonstraled a det＇rease in strength followed hy a gradual increase. The average gastroenemius muscle strength of the rats in the BTX-A and P-NCAM-Ah groups was signi｜＇i~＇anlty lower than that of the control group at all time points. Compared with the BTX-A group, the muscle strength of the P-N（＇AM-Ab group rats decreased further. Strength recox＇ery in Ihe BTX-A and P-NCAM-Ah groups was signil＇i~＇antly slower lha,, in the co,tirol group. The wet weight percentage in the BTX-A and P-NCAM-Ah grut, ps at I＇i~＇s/ decreased and then recovered wilh time. Alter the BTX-A injection, the average wet weight pe,＇eentage of Ihe P-NCAM-Ah group rats was signifil~antly lower than that of the BTX-A group after 3 clays, and I , 2 a