中文摘要：

由白粉病菌（Blumeria graminisf.sp.tritici）引起的小麦白粉病是严重影响小麦安全生产的主要病害之一。本研究将来自以色列的野生二粒小麦（Triticum dicoccoides）WE27的坏白粉病基因通过杂交和连续回交,导入普通小麦遗传背景中,育成高抗白粉病小麦新品系3D256（其系谱为燕大1817/WE27//农大015/3/941,F6）。将3D256和高感小麦白粉病的普通小麦品系薛早配制杂交组合,对其F1、F2分离群体和F3家系进行白粉病抗性鉴定和遗传分析。结果表明,3D256携带抗白粉病显性单基因,暂命名为MlWE27。利用集群分离分析法（BSA）和分子标记分析,发现3个SSR标记（Xwmc243、Xwmc154和Xbarc318）、1个EST-SSR标记（Xdp357）、1个AFLP转化的SCAR标记（XCAUG1）和1个RFLP探针转化的STS标记（XWG516-1）与抗白粉病基因MlWE27连锁,在连锁图上的顺序为Xdp357-MlWE27-XCAUG1-XWG516-1-Xwmc243-Xwmc154-Xbarc318。利用中国春缺体-四体系、双端体系和缺失系将抗白粉病基因MlWE27定位于染色体2B短臂的末端Bin0.84-1.00上。这一普通小麦抗白粉病种质资源的创制及其连锁分子标记的建立为小麦抗病基因分子标记辅助选择、基因积聚和分子育种提供了新的物质基础。

英文摘要：

Powdery mildew,caused by Blumeria graminis f.sp.tritici,is one of the most important wheat diseases worldwide.The common wheat（Triticum aestivum） line 3D256,a derivative of wild emmer（Triticum dicoccoides） with pedigree Yanda1817/WE27//Nongda015/3/941,is highly resistant to prevailing local wheat powdery mildew isolate E09 in Beijing area.Genetic analysis of the F2 population and their F3 lines,developed from 3D256 and a susceptible common wheat line Xuezao,indicated that the powdery mildew resistance in 3D256 was controlled by a single dominant gene,designated temporarily MlWE27.Molecular markers and the bulked segregant analysis were used to characterize the powdery mildew resistance gene MlWE27.Three SSR markers（Xwmc243、Xwmc154 and Xbarc318）,one EST-SSR marker（Xdp358）,one AFLP-derived SCAR markers（XCAUG1） and one RFLP-derived STS marker（XWG516-1） were found to be linked to the powdery mildew resistance gene MlWE27,with an order of Xdp357-MlWE27-XCAUG1-XWG516-1-Xwmc243-Xwmc154-Xbarc318 in the genetic linkage map.Using Chinese Spring nullisomic-tetrasomics,ditelosomics and deletion lines,MlWE27 was physically mapped on chromosome 2BS terminal bin 0.84-1.00.The new developed common wheat powdery mildew resistance line 3D256 and its resistance gene linked molecular markers provide useful germplasm and tool in disease resistance genes pyramiding and marker-assisted selection（MAS） in wheat breeding program.