中文摘要：

目的：利用幼年间歇缺氧大鼠模型,研究胰岛素样生长因子-1（IGF-1）及胰岛素样生长因子结合蛋白-3（IGFBP-3）在肝脏中的表达,试图为OSAHS患儿可能存在的生长发育迟缓提供客观依据。方法：将24只大鼠随机分为3组,即对照组及轻、重度低氧组,对照组正常喂养,不加处理,将其他2组分别饲养于已模拟符合儿童OSAHS轻、重度条件的间歇缺氧箱内。每天循环间歇缺氧8h,共35d。至实验最后1d,处死取新鲜肝脏组织于液态氮固定,并置于-80℃冰箱冻存,进行IGF-1及IGFBP-3的分子生物学实验。结果：肝脏中IGF-1的mRNA表达半定量分析显示,3组之间差异有统计学意义（P〈0.05）,两两比较,轻度缺氧组高于对照组及重度缺氧组,差异有统计学意义（均P〈0.05）,对照组与重度缺氧组差异无统计学意义（P〉0.05）。IGFBP-3的mRNA表达半定量分析显示,3组之间差异无统计学意义（P〉0.05）。IGF-1在肝脏组织中表达量分析显示,3组之间差异有统计学意义（P〈0.05）,两两比较,对照组低于轻度缺氧组及重度缺氧组,差异有统计学意义（P〈0.05）;轻度缺氧组与重度缺氧组差异无统计学意义（P〉0.05）。IGFBP-3在肝脏中的表达量,3组之间差异有统计学意义（P〈0.05）;两两比较,对照组高于轻度缺氧组及重度缺氧组,差异有统计学意义（P〈0.05）;轻度缺氧组与重度缺氧组差异无统计学意义（P〉0.05）。结论：轻度缺氧组、重度缺氧组及对照组间IGF-1及IGFBP-3在肝脏中的转录与翻译水平与缺氧严重程度无明显关系。

英文摘要：

Objective：Using the model of young rats with intermittent hypoxia,to study the expression of insulin-like growth factor-1（IGF-1）and insulin-like growth factor binding protein-3（IGFBP-3）in liver,and to provide objective evidence for the possible existence of growth retardation in children with OSAHS.Method：Divided 24 rats randomly into three groups,as control group and the mild and severe hypoxia group.Normally fed control group,without treatment,the other two groups were kept in the cabin has been simulated mild and severe intermittent hypoxia conditions of OSAHS in children.8-hour day cycle of intermittent hypoxia,a total of 35 days.To the last day,animals were sacrificed and fresh liver tissues were fixed in liquid nitrogen,stored in the-80 Crefrigerator,then took the molecular biology experiment of IGF-1 and IGFBP-3 in the liver.Result：The expression of IGF-1mRNA in liver,semi-quantitative analysis showed that the difference of mRNA expression of IGF 1 among the three groups was statistically significant（P〈0.05）,pairwise comparison,mild hypoxia group was higher than control and severe group,there were significant differences（P〈0.05）,the control group and severe hypoxia had no significant difference（P〈0.05）.The expression of IGFBP-3mRNA in liver showed that,differences between the three groups was not significant（P 0.05）.IGF-1 expression in liver tissue analysis showed that,differences between the three groups was statistically significant（P〈0.05）,pairwise comparisons,its expression in the control group was lower than mild hypoxia group and severe group,there were significant differences（P〈0.05）,mild hypoxia and severe hypoxia group had no significant difference（P 0.05）.IGFBP-3expression in liver tissue analysis showed that,differences between the three groups was statistically significant（P〈0.05）,pairwise comparisons,the control group was higher than mild and severe hypoxic group,there were significant differences（P〈0.05）,mild hypoxia and severe h